三氧化二砷和顺铂联用对乳腺癌裸鼠移植瘤生长抑制作用

目的 观察三氧化二砷(As2O3)和顺铂(DDP)联用对人乳腺癌细胞系MCF-7裸鼠移植瘤生长抑制有无增效作用.方法 建立裸鼠人乳腺癌MCF-7细胞移植瘤动物模型,成瘤后动物随机分为4组:对照组、As2O3组、DDP组、As2O3+DDP组.对照组予0.9%氯化钠注射液0.2 mL,隔日1次腹腔注射,共8次.As2O3...

Inhibition of combined application of arsenic trioxide and cisplatin on the growth of MCF-7 xenografted tumors in mude mice

Objective To study whether arsenic trioxide could potentiate the anti-tumor effect of cisplatin（DDP） on xenografted tumor growth of human breast cancer cell line MCF-7 in nude mice.Methods Human breast cancer cells（MCF-7 cells） were transplanted subcutaneously to BALB/C nude mice to generate tumors.Twenty-four nude mice bearing human breast cancer MCF-7 xenografted tumor were randomly divided into 4 groups,control group,arsenic trioxide group,cisplatin group and combination group.The model mice in four groups were respectively given 0.9% NaCl solution,3.0 mg/kg As2O3,0.2 mL once two days,3.0 mg/kg DDP,0.2 mL twice a week,and combination of 1.5 mg/kg As2O3,0.2 mL once two days and 1.5 mg/kg DDP,0.2 mL twice a week.The tumor volumes and weight were measured respectively during the treatment.Tumor growth inhibition rate was calculated.Transmission electron microscopy was used to detect cellular ultrastructure of implanted tumor cells.Results The tumor weight and volume of the combination group were significantly different from those of other 3 groups（P0.05）.The nuclear fragmentation,chromatin condensation and margination occurred in combination group.Conclusion Arsenic trioxide and cisplatin can significantly inhibit tumor growth in nude mice bearing human breast cancer cell line MCF-7 and their combination can produce a synergistic action.