Sub-chronic sulforaphane exposure in CD-1 pregnant mice enhances maternal NADPH quinone oxidoreductase 1 (NQO1) activity and mRNA expression of NQO1, glutathione S-transferase,and glutamate-cysteine ligase: Potential implications for fetal protection against toxicant exposure |
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| Affiliation: | 1. Department of Biomedical and Molecular Sciences, Graduate Program in Pharmacology and Toxicology Graduate Program, Queen''s University, Kingston, Ontario, Canada K7L 3N6;2. School of Environmental Studies, Queen''s University, Kingston, Ontario, Canada K7L 3N6;1. Food and Nutritional Sciences Programme, School of Life Sciences, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong;2. Biochemistry Programme, School of Life Sciences, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong;1. National Reference Laboratory of Veterinary Drug Residues (HZAU) and MAO Key Laboratory for Detection of Veterinary Drug Residues, Wuhan, Hubei 430070, China;2. MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Huazhong Agricultural University, Wuhan, Hubei 430070, China;3. Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan, Hubei, China;4. Department of Biosciences, COMSATS Institute of Information Technology, Sahiwal, Pakistan;1. Division of Cardiology, Department of Medicine, Hôpital du Sacré-Coeur de Montréal, Université de Montréal, Monreal, Quebec, Canada;2. Division of Non Invasive Cardiology, Department of Medicine, Montreal Heart Institute, Université de Montréal, Montreal, Quebec, Canada;3. Division of Cardiac Surgery, Department of Surgery, Hôpital du Sacré-Coeur de Montréal, Université de Montréal, Montréal, Quebec, Canada;1. Department of Obstetrics and Gynecology, Shandong University, Qilu Hospital, No. 104 Culture West Road, Jinan, 250012, Shandong, China;2. Department of Obstetrics and Gynecology, Liaocheng People’s Hospital, No.67 of Dongchang West Road, Liaocheng, 252000, Shandong, China;3. Drug Clinical Trial Institution, Liaocheng People’s Hospital, No.67 of Dongchang West Road, Liaocheng, 252000, Shandong, China |
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| Abstract: | The study objective was to determine if maternal administration of sulforaphane (SFN) induced Nrf2-controlled genes. In acute studies, when non-pregnant and pregnant mice were orally exposed to SFN (50 or 100 mg/kg) on gestational day (GD) 14 and euthanized after 2, 6 or 24 h, results demonstrated increased GSTM1, NQO1, HO-1, and Gclc mRNA transcript levels in adult liver, but no change in NQO1 activity. In sub-chronic studies, when non-pregnant and pregnant mice were orally exposed to SFN (65 mg/kg) daily for 30 days and euthanized on GD14, results demonstrated a 2- to 3-fold increase in GSTM1, Gclc and NQO1 transcript levels, and a 2-fold increase in NQO1 activity in adult livers. No effects of maternal treatment on fetal liver gene transcript levels or enzyme activity were observed. Demonstration that SFN induces maternal gene expression and activity supports further investigation of SFN as a preventative agent against transplacental toxicity. |
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| Keywords: | Sulforaphane GST HO-1 Gclc NQO1 |
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