Environmental pH influences Candida albicans biofilms regarding its structure,virulence and susceptibility to fluconazole |
| |
| Affiliation: | 1. Department of Prosthodontics and Periodontology, Piracicaba Dental School, University of Campinas, Av. Limeira, 901, 13414-903 Piracicaba, São Paulo, Brazil;2. Faculty of Dentistry, Federal University of Ceará, Sobral Campus, Av. Coronel Estanislau Frota, s/n, 62010-560 Sobral, Ceará, Brazil;3. Post-Graduate Program in Dentistry, CEUMA University, Josué Montello, 1, 65075-120 São Luís, Maranhão, Brazil;1. Department of Gerontology, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, China;2. Department of Rheumatology, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, China;3. Department of Cardiology, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, China;1. Department of Biology, University of Pisa, Pisa, Italy;2. Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Pisa, Italy;3. National Center for Drug Research and Evaluation, Italian National Institute of Health, Rome, Italy;4. Department of Infectious Diseases, Italian National Institute of Health, Rome, Italy;5. Advanced Light and Electron Microscopy, Robert Koch Institute, Berlin, Germany;6. Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA |
| |
| Abstract: | Candida albicans colonizes sites with different environmental pH. However, it is unclear how these conditions can interfere on biofilms. This study aimed to evaluate the influence of environmental pH on behavior of C. albicans regarding its structure, virulence and susceptibility to fluconazole (FLZ). Minimal inhibitory concentration, minimal fungicidal concentration and time kill were used to evaluate the susceptibility to FLZ in planktonic cells under three pH values (4.0, 5.5, 7.0). These pH values were used for biofilms analysis. C. albicans ATCC 90028 was developed on poly(methlymethacrylate) resin for 48 h. Then, 2.56 μg/mL of FLZ was added to experimental groups for 24 h, and biofilms were analyzed by cell quantification, bioactivity, secretion of proteinases and phospholipases and structure. All data were analyzed by two-way ANOVA, followed by Tukey's test (α = 0.05). For planktonic cells, changes in environmental pH decreased the susceptibility to FLZ. C. albicans biofilms developed at pH 5.5 showed higher cell counts, bioactivity, bio-volume, average thickness and roughness coefficient (p < 0.05). In contrast, the presence of FLZ at pH 4.0 did not influence the structural parameters (p > 0.05), but increased secretion of proteinase and phospholipase (p < 0.05). Within the conditions studied, it was shown that environmental pH modulates the structure, virulence and susceptibility of C. albicans to FLZ. |
| |
| Keywords: | antifungal activity susceptibility |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
