Calcium-binding proteins in the laterodorsal thalamic nucleus during development of the guinea pig |
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| Institution: | 1. Department of Medical Neuroscience and Neurosurgery, College of Medicine, Chungbuk National University, Cheongju, Republic of Korea;2. Department of Radiology, Daejeon St. Mary’s Hospital, The Catholic University of Korea, Republic of Korea;3. Department of Neurosurgery, Saint Vincent’s Hospital, The Catholic University of Korea, Republic of Korea;4. Department of Neurosurgery, Chungbuk National University Hospital, Chungbuk National University, College of Medicine, Cheongju, Republic of Korea;1. Department of Neurology, Boston University School of Medicine, Boston, MA, USA;2. The Framingham Heart Study, Framingham, MA, USA;3. Lille University, Inserm, Lille University Hospital, Institut Pasteur de Lille, U1167 - RID-AGE - Risk factors and molecular determinants of aging-related diseases, Labex Distalz, Lille, France;4. Department of Biostatistics, Boston University School of Public Health, Boston, MA, USA;5. School of Public Health, University of Haifa, Israel;6. Division of Cardiovascular Medicine, Vanderbilt Heart & Vascular Institute, Nashville, TN, USA;7. Cardiology Division, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA;8. Broad Institute of MIT and Harvard, Cambridge, MA, USA;9. Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA;10. Department of Medicine (Sections of Preventive Medicine and Cardiology), Boston University School of Medicine, Boston, MA, USA;1. Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran;2. NeuroBiology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran;1. Department of Human Anatomy, Histology and Embryology & K. K. Leung Brain Research Centre, Preclinical School of Medicine, The Fourth Military Medical University, Xi’an 710032, China;2. Department of Orthopedics, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, China;1. The Mind Research Network, 1101 Yale Blvd. NE, Albuquerque, NM 87106, United States;2. Tri-Institutional Center for Translational Research in Neuroimaging and Data Science (TReNDS), Georgia State University, Georgia Institute of Technology, and Emory University, 55 Park Place NE, Atlanta, GA 30303, United States;3. Department of Neurosciences, University of New Mexico School of Medicine, 1 University of New Mexico, Albuquerque, NM 87131, United States;4. Department of Psychology, University of New Mexico, 1 University of New Mexico, Albuquerque, NM 87131, United States |
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| Abstract: | The laterodorsal thalamic nucleus (LD) is often treated as a part of the anterior thalamic nuclei (ATN) because of its location and similar connectivity. Our previous studies have shown that distribution of three calcium-binding proteins, i.e. calbindin D28k (CB), calretinin (CR) and parvalbumin (PV), changes within the ATN during development of the guinea pig. The aim of this study is to examine the immunoreactivity pattern of these proteins in the LD in the guinea pig ontogeny. Brains from animals ranging from 40th embryonic day to 80th postnatal day were used in the study. Two methods were applied: a single-labelling immunoenzymatic method and double-labelling immunofluorescence. No changes of the distribution pattern of the substances were observed throughout the examined developmental stages. CB and CR were the most abundantly expressed proteins in perikarya of the LD. Numerous CB- and CR-immunoreactive cell bodies were found throughout the whole extent of the nucleus. In most of these cell bodies both proteins colocalized vastly. The highest immunoreactivity of the perikarya containing CB and CR was observed in the mediodorsal part of the LD and in its rostral portion. In regard to PV, single cell bodies were observed mostly in the dorsal part of the nucleus. PV did not colocalize with the other proteins. In summary, all the studied calcium-binding proteins were already present in the LD at prenatal developmental stages and the pattern of distribution remained virtually constant until adulthood. Thus, the LD differs considerably from the ATN in an aspect of neurochemical cell differentiation. |
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| Keywords: | Laterodorsal thalamic nucleus Calbindin Calretinin Parvalbumin Development Guinea pig |
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