Different metabolic profiles of K1 serotype and non-serotype K1 and K2 Klebsiella pneumoniae isolates in oral infection mice model |
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| Affiliation: | 1. Department of Medical Microbiology, Medicine College, Hebei University, Baoding 071000, China;2. Department of Basic Medical Sciences, Zhejiang University School of Medicine, Hangzhou 310058, China;3. Basic Medical Sciences, Medicine College, Hebei University, Baoding 071000, China;4. Department of Pharmacology, Zhejiang Medical College, Hangzhou 310053, China;1. Dept. of Immunology and Molecular Medicine, Sher-i-Kashmir Institute of Medical Sciences (SKIMS), Soura, Srinagar, Kashmir, J&K 190011, India;2. Dept. of Gastroenterology, Sher-i-Kashmir Institute of Medical Sciences (SKIMS), Soura, Kashmir, J&K 190011, India;3. Dept. of General Surgery, Sher-i-Kashmir Institute of Medical Sciences (SKIMS), Soura, Kashmir, J&K 190011, India;1. Aix-Marseille University, Unité de Recherche sur les Maladies Infectieuses Transmissibles et Emergentes, UMR 63, CNRS 7278, IRD 198, INSERM U1095, Marseille, France;2. Inserm UMR 1068, Centre de Recherche en Cancérologie de Marseille, Marseille, France;1. Nucleo de Medicina Tropical, Universidade Federal do Para, Belem, PA, Brazil;2. Centro de Ciencias Biologicas e da Saude, Universidade do Estado do Para, Belem, PA, Brazil;3. Departamento de Patologia, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, Brazil;1. Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 1374, São Paulo, SP 05508-900, Brazil;2. Department of Endodontics, School of Dentistry, University of São Paulo, Av. Prof. Lineu Prestes, 2227, São Paulo, SP 05508-900, Brazil;3. Department of Electron Microscopy, Adolfo Lutz Institute, Av Dr. Arnaldo, 355, São Paulo, SP 01246-902, Brazil;1. Department of Pathogenic Biology, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China;2. Department of Gastroenterology, Second Hospital of Gansu Province, Lanzhou 730000, China;1. Department of Microbiology, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan;2. Laboratory of Hygienic Chemistry, College of Pharmaceutical Sciences, Matsuyama University, 4-2 Bunkyo-cho, Matsuyama, Ehime 790-8578, Japan;3. Department of Analytical Chemistry, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan |
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| Abstract: | K1 or K2 serotype Klebsiella pneumoniae isolate caused clinical pyogenic liver abscess (KLA) infection is prevalent in many areas. It has been identified that K1 or K2 serotype K. pneumoniae isolates caused KLA infection in mice by oral inoculation. In our study, K1 serotype K. pneumoniae isolate Kp1002 with hypermucoviscosity (HV)-positive phenotype caused KLA infection in C57BL/6 mice by oral inoculation. Simultaneously, non-serotype K1 and K2 isolate Kp1014 with HV-negative phenotype failed to cause KLA infection in the same manner. It seems that gastrointestinal tract translocation is the pathway by which K1 or K2 serotype K. pneumoniae caused KLA infection. Liquid chromatography-tandem mass spectrometry was used to further analyze metabolic profile changes in mice with KLA infection. Data showed that after Kp1002 or Kp1014 oral inoculation, serum Phosphatidylcholine (PC) and Lysophosphatidylcholine (LPC) levels significantly changed in mice. Some PC and LPC molecules showed changes both in the Kp1002 KLA group and the Kp1014 no-KLA group compared with the control group. The level of 18:1/18:2-PC significantly changed in the Kp1002 KLA group compared with the control group, but showed no change between the Kp1014 no-KLA group and the control group. The level of 18:1/18:2-PC might have been particularly affected by KLA infection caused by K1 serotype K. pneumoniae Kp1002. It may be a potential biomarker for KLA infection. |
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| Keywords: | Liver abscess Metabolomics LC-MS Gastrointestinal tract translocation PLA" },{" #name" :" keyword" ," $" :{" id" :" kwrd0040" }," $$" :[{" #name" :" text" ," _" :" Pyogenic liver abscess KLA" },{" #name" :" keyword" ," $" :{" id" :" kwrd0050" }," $$" :[{" #name" :" text" ," $$" :[{" #name" :" italic" ," _" :" Klebsiella pneumoniae" },{" #name" :" __text__" ," _" :" -caused pyogenic liver abscess HV" },{" #name" :" keyword" ," $" :{" id" :" kwrd0060" }," $$" :[{" #name" :" text" ," _" :" Hypermucoviscosity QC" },{" #name" :" keyword" ," $" :{" id" :" kwrd0070" }," $$" :[{" #name" :" text" ," _" :" Quality control LC-MS" },{" #name" :" keyword" ," $" :{" id" :" kwrd0080" }," $$" :[{" #name" :" text" ," _" :" Liquid chromatography-mass spectrometry LC MS/MS" },{" #name" :" keyword" ," $" :{" id" :" kwrd0090" }," $$" :[{" #name" :" text" ," _" :" Liquid chromatography-tandem mass spectrometry PCA" },{" #name" :" keyword" ," $" :{" id" :" kwrd0100" }," $$" :[{" #name" :" text" ," _" :" Principal component analysis PLS-DA" },{" #name" :" keyword" ," $" :{" id" :" kwrd0110" }," $$" :[{" #name" :" text" ," _" :" Partial least-squares discriminant analysis OPLS-DA" },{" #name" :" keyword" ," $" :{" id" :" kwrd0120" }," $$" :[{" #name" :" text" ," _" :" Orthogonal partial least-squares discriminant analysis ALT" },{" #name" :" keyword" ," $" :{" id" :" kwrd0130" }," $$" :[{" #name" :" text" ," _" :" Alanine aminotransferase AST" },{" #name" :" keyword" ," $" :{" id" :" kwrd0140" }," $$" :[{" #name" :" text" ," _" :" Aspartate Aminotransferase VIP" },{" #name" :" keyword" ," $" :{" id" :" kwrd0150" }," $$" :[{" #name" :" text" ," _" :" Variable importance in the projection HILIC" },{" #name" :" keyword" ," $" :{" id" :" kwrd0160" }," $$" :[{" #name" :" text" ," _" :" Hydrophilic interaction chromatography RPLC" },{" #name" :" keyword" ," $" :{" id" :" kwrd0170" }," $$" :[{" #name" :" text" ," _" :" Reversed-phase liquid chromatography HPLC-MC" },{" #name" :" keyword" ," $" :{" id" :" kwrd0180" }," $$" :[{" #name" :" text" ," _" :" High-performance liquid chromatography-mass spectrometry PC" },{" #name" :" keyword" ," $" :{" id" :" kwrd0190" }," $$" :[{" #name" :" text" ," _" :" Phosphatidylcholine LPC" },{" #name" :" keyword" ," $" :{" id" :" kwrd0200" }," $$" :[{" #name" :" text" ," _" :" Lysophosphatidylcholine |
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