Effects and potential mechanisms of Danzhi Xiaoyao Pill (丹栀逍遥丸) on proliferation of MCF-7 human breast cancer cells in vitro

Objective： To investigate the effects of 50% ethyl alcohol （EtOH） extracts from Danzhi Xiaoyao Pill （丹栀逍遥丸, DXP） on the proliferation of MCF-7 human breast cancer cells and potential mechanisms. Methods： ATP-Lite assay was performed to test the proliferation of the MCF-7 breast cancer cell line; and antioxidant activity was measured by the oxygen radical absorbance capacity （ORAC）. The effects of DXP on nitric oxide （NO） production were tested by lipopolysaccharide （LPS）- stimulated RAW 264.7 murine macrophages using the Griess reaction. Results： The 50% EtOH DXP extracts displayed a cytotoxic response on MCF-7 cells at 0.10, 0.25 and 0.50 mg/mL dosedependently with the proliferation inhibited by more than 85%. The ORAC value of the DXP was 820μ moL Trolox equivalent/g, about 40% of the vitamin C value. DXP extracts had significant inhibitory effect on NO production at the concentration from 0.0625 mg/mL to 0.5 mg/mL （P〈0.05, P〈0.01）. Conclusion： The extracts of DXP could significantly inhibit the proliferation of MCF-7 cells, with the effect possibly related to its antioxidant activity and the inhibition of NO production.

Effects and potential mechanisms of Danzhi Xiaoyao Pill on proliferation of MCF-7 human breast cancer cells in vitro

Objective:To investigate the effects of 50% ethyl alcohol(EtOH)extracts from Danzhi mechanisms.Methods:ATP-Lite assay was performed to test the proliferation of the MCF-7 breast cancer cell line;and antioxidant activity was measured by the oxygen radical absorbance capacity (ORAC).The effects of DXP on nitric oxide(NO)production were tested by lipopolysaccharide(LPS)-stimulated RAW 264.7 murine macrophages using the Griess reaction.Results:The 50% EtOH DXP extracts displayed a cytotoxic response on MCF-7 cells at 0.10,0.25 and 0.50 mg/mL dosedependently with the proliferation inhibited by more than 85%.The ORAC value of the DXP was 820 μ moL Trolox equivalent/g.about 40% of the vitamin C value.DXP extracts had significant inhibitory effect on NO production at the concentration from 0.0625 mg/mL to 0.5 mg/mL(P＜0.05,P＜0.01).Conclusion:The extracts of DXP could significantly inhibit the proliferation of MCF-7 cells,with the effect possibly related to its antioxidant activity and the inhibition of NO production.