氨茶碱及地塞米松对小鼠脾淋巴细胞凋亡的调节作用

目的：观察氨茶碱和地塞米松对小鼠脾淋巴细胞凋亡的调节作用。方法：采用流式细胞技术对比了它们对正常鼠及卵蛋白致敏小鼠脾淋巴细胞凋亡的作用。结果：氨茶碱及地塞米松可诱导体外培养的正常小鼠淋巴细胞凋亡，明显抑制体外培养的致敏鼠淋巴细胞的凋亡。致敏鼠淋巴细胞的凋亡明显多于对照鼠，在体应用氨茶碱（１００ｍｇ／ｋｇ）使致敏淋巴细胞凋亡进一步增加，而在体应用地塞米松（４０ｍｇ／ｋｇ）明显抑制致敏鼠淋巴细胞的凋亡。氨茶碱体外对在体应用氨茶碱处理的鼠淋巴细胞的凋亡有抑制作用，地塞米松体外对在体应用地塞米松处理的鼠淋巴细胞的凋亡无明显影响。结论：氨茶碱和地塞米松对淋巴细胞凋亡具有重要的调节作用，两者的作用机制可能有别。

Modulating effect of aminophylline and dexamethasone on splenic lymphocyte apoptosis

AIM and METHODS:It is known that aminophyline (AP) and Dexamethasone (Dex) can inhibit immune system in vivo and in vitro . In this study flow cytometry was used to study the effects of AP and Dex on splenic lymphocyte apoptosis in control and ovalbumin (OA)-sensitized mice. RESULTS:AP and Dex induce lymphocyte apoptosis in control mice, but inhibit lymphocyte apoptosis in OA-sensitized mice in vitro . The apoptosis rate was higher in OA-sensitized group than in the control group. AP application in vivo (100 mg/kg) enhanced the apoptosis of OA-sensitized lymphocytes, while Dex(40 mg/kg) inhibited the apoptosis of OA-sensitized lymphocytes. AP in vitro also inhibited the apoptosis of AP-pretreated lymphocytes. Dex did not have any effect on the apoptosis of Dex-pretreated lymphocytes.CONCLUSION:both AP and Dex play an important part in modulating lymphocyte apoptosis, possibaly via different machanism.