| 外环境水样中杂菌对不同型别霍乱弧菌分离培养干扰情况的研究 |
| |
| 引用本文： | 徐海滨,陈爱平,林杰,杨劲松,陈建辉,董新平. 外环境水样中杂菌对不同型别霍乱弧菌分离培养干扰情况的研究[J]. 中国卫生检验杂志, 2011, 0(8): 1925-1927 |
| |
| 作者姓名： | 徐海滨 陈爱平 林杰 杨劲松 陈建辉 董新平 |
| |
| 作者单位： | 福建省疾病预防控制中心; |
| |
| 基金项目： | 福建省卫生厅青年科研课题(2008-2-10) |
| |
| 摘 要： | 目的:了解外环境水样中存在的杂菌对不同型别的霍乱弧菌分离培养的干扰情况。方法:采用稀释法分别将已用巢式PCR证实不含O1和O139群VC及含有ctxA阴性的O139群VC野生株的外环境水样一代、二代增菌液对不同型别的霍乱弧菌进行倍比稀释,观察霍乱弧菌在庆大霉素琼脂平皿上的生长情况。结果:不同厂家购买的庆大平板对霍乱弧菌生长的抑制能力有所不同,A厂家购买的庆大琼脂对霍乱弧菌本身生长的抑制力要差些。用一代增菌液为稀释介质时,CT-的霍乱菌株比CT+的菌株容易检出,同时CT-的小川型VC生长繁殖能力在实验用的不同型别的霍乱弧菌中是最强的。结论:无论是否存在有O139群VC野生株干扰时,以一代增菌液为稀释介质时目的霍乱弧菌均更易于检出。二代增菌液中存在的杂菌对霍乱弧菌分离培养干扰要比一代增菌液严重,更不利于霍乱弧菌的检出。
|
| 关 键 词： | 外环境水样 霍乱弧菌 分离培养 |
Study on the interference of miscellaneous bacteria on the culture and isolation of Vibrio cholerae in different types in environmental water sample |
| |
| XU Hai-bin,CHEN Ai-ping,LIN JIe,Yang Jing-song,CHEN Jian-hui,DONG Xin-ping. Study on the interference of miscellaneous bacteria on the culture and isolation of Vibrio cholerae in different types in environmental water sample[J]. Chinses Journal of Health Laboratory Technology, 2011, 0(8): 1925-1927 |
| |
| Authors: | XU Hai-bin CHEN Ai-ping LIN JIe Yang Jing-song CHEN Jian-hui DONG Xin-ping |
| |
| Affiliation: | XU Hai-bin,CHEN Ai-ping,LIN JIe,Yang Jing-song,CHEN Jian-hui,DONG Xin-ping(Fujian Center for Disease Control and Prevention,Fuzhou 350001,China) |
| |
| Abstract: | Objective:To investigate the interference of miscellaneous bacteria on the culture and isolation of different types of Vibrio cholerae in environmental water sample.Methods:To observe the growth on gentamicin agar after different types of V.cholera were diluted by first generation and second generation enrichment broth of environmental water sample without O1 VC or O139 VC,and with ctxA-negative VC O139 wild strains which have been confirmed by nested PCR.Results:The ability of gentamicin agar from differen... |
| |
| Keywords: | Environmental water samples Vibrio cholerae Isolation and culture |
| 本文献已被 CNKI 等数据库收录！ |
|
