| 多功能柱净化-光化学柱后衍生高效液相色谱测定食品中黄曲霉毒素 |
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| 引用本文： | 吴燕,朱怡平,汪国权. 多功能柱净化-光化学柱后衍生高效液相色谱测定食品中黄曲霉毒素[J]. 中国卫生检验杂志, 2011, 0(8) |
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| 作者姓名： | 吴燕 朱怡平 汪国权 |
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| 作者单位： | 上海市疾病预防控制中心; |
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| 摘 要： | 目的:研究建立了光化学柱后衍生-高效液相色谱-荧光检测器测定食品中黄曲霉毒素B1、B2、G1和G2的方法。方法:乙腈/水提取样品中黄曲霉毒素B1、B2、G1和G2,经多功能柱净化、高效液相色谱分离、光化学柱后衍生,荧光检测器测定。结果:黄曲霉毒素B1、B2、G1和G2的检出限分别为0.50μg/kg、0.15μg/kg、0.50μg/kg和0.15μg/kg,回收率为86.7%～97.2%,相对标准偏差(RSD)0.41%～2.40%。结论:该方法简便快速、灵敏度高、重现性好,满足食品中黄曲霉毒素检测的需要。
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| 关 键 词： | 多功能净化柱 光化学衍生 高效液相色谱 黄曲霉毒素 食品 |
Determination of aflatoxin B_1,B_2,G_1,G_2 in food by multifunctional clean-up column,post-column photochemical derivatization and HPLC |
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| WU Yan,ZHU Yi-ping,WANG Guo-quan. Determination of aflatoxin B_1,B_2,G_1,G_2 in food by multifunctional clean-up column,post-column photochemical derivatization and HPLC[J]. Chinses Journal of Health Laboratory Technology, 2011, 0(8) |
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| Authors: | WU Yan ZHU Yi-ping WANG Guo-quan |
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| Affiliation: | WU Yan,ZHU Yi-ping,WANG Guo-quan(Shanghai Municipal Center for Disease Prevention and Control,Shanghai 200336,China) |
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| Abstract: | Objective:A method for determination of Aflatoxin B1,B2,G1 and G2 by using post-column photochemical derivatization-HPLC-fluorescence detector was developed.Methods:The Aflatoxin B1,B2,G1 and G2 in samples were extracted by acetonitrile/water and were cleaned up by multifunctional clean-up columns.The separation of Aflatoxin B1,B2,G1 and G2 was conducted by HPLC,the determination was carried out by fluorescence detector after photochemical derivatization.Results:The detection limits of Aflatoxin B1,B2,G1 an... |
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| Keywords: | Multifunctional clean-up column Photochemical derivatization High performance liquid chromatography Aflatoxin food |
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