小分子肽产生菌培养基碳源与氮源的优化

目的:探讨不同碳源、氮源对乳酸杆菌小分子肽产量及抑菌效果的影响。方法:用1%的不同碳源和2%的不同氮源替换基础培养基中的葡萄糖、酵母膏和蛋白胨,同时改变基础培养基中的葡萄糖浓度,分别在相同条件下发酵,双层平板法检测抑菌效果,同时测定发酵液的OD值和PH值。结果:不同碳源均能促进LB-9菌株的生物量增加,生物量从高到低依次为葡萄糖、果糖、蔗糖、乳糖、木糖、糊精、玉米浆、可溶性淀粉,其中葡萄糖为碳源的发酵液的生物量最大。各有机氮源均可促进小分子肽的产生,而无机氮源不能。有机氮源发酵液的抑菌效果从高到低依次为酵母膏、牛肉膏、多胨、鱼蛋白胨、胰蛋白胨、大豆胨。结论:葡萄糖是本实验最好的碳源。葡萄糖为碳源的发酵液的生物量最大,小分子肽产量随葡萄糖浓度的增加而增加。菌体细胞生长最好的氮源是酵母膏,有机氮源均可促进小分子肽的产生,而无机氮源不能。

Optimization of carbon sources and nitrogen sources in ferment culture medium of bacterium producing small molecular antibacterial peptides

Objective:To investigate the effect of different carbon and nitrogen sources for inhibition activity of small molecular antibacterial peptides producing by lactobacillus in ferment culture medium.Methods:To substitute glucose,yeast cream and peptone of seed culture medium using different 1% carbon sources and 2% nitrogen sources respectively.PH,OD and inhibition activity of culture medium were tested.Results:The different carbon sources can improve the amount of antibacterial substance producing by strain L...