氟化钠对小鼠的急性毒性和致染色体突变作用研究

目的:研究氟化钠对小鼠的急性毒性及致染色体突变作用。方法:采用急性毒性试验和小鼠骨髓微核试验。设35.0 mg/kg、55.56 mg/kg8、8.18 mg/kg1、39.97 mg/kg2、22.18 mg/kg3、52.67 mg/kg5、59.79 mg/kg 7个剂量组NaF,观察氟化钠的急性毒性,计算经口半数致死量(LD50);另设高(34 mg/kg)、中(17 mg/kg)、低(8.5 mg/kg)3个剂量组NaF及环磷酰胺阳性对照组(50 mg/kg)、生理盐水空白对照组,观察小鼠骨髓细胞微核率的变化。结果:急性毒性实验各剂量组中毒小鼠均出现不同程度的抽搐、中枢神经系统先兴奋后抑制等表现,氟化钠经口LD50为168.34 mg/kg;氟化钠能诱变小鼠骨髓细胞微核率增高,中、高剂量组微核率均明显高于空白对照组(P<0.01),低剂量组微核率与空白对照组相比,差别无统计学意义(P>0.05)。结论:氟化钠属中等毒性,高浓度氟有致突变作用,能引起染色体损伤,具有潜在的遗传毒性效应。

The acute toxicity and mutagenic effect of sodium fluoride in mice

Objective:The aim of the study was to determine the acute toxicity of sodium fluoride on mice and to investigate its mutagenic effect in bone marrow cell chromosome.Methods:Acute toxicity test and micronucleus test of bone marrow in mice were used in this study.In the first set of experiments,mice were given sodium fluoride at doses of 35.0 mg/kg,55.56 mg/kg,88.18 mg/kg,139.97 mg/kg,222.18 mg/kg,352.67 mg/kg,559.79 mg/kg respectively.Mice were observed for 7 days after sodium fluoride.The median lethal dose...