当归多糖对小鼠骨髓基质细胞增殖、细胞周期及细胞凋亡的影响

目的研究当归多糖（APS）对小鼠骨髓基质细胞（BMSC）增殖及细胞周期和细胞凋亡的影响。方法原代培养BMSC，随机分为对照组和药物组，作用不同时间后观察APS浓度为50mg／L、100mg／L时对小鼠BMSC80％贴壁时间的影响，MTT法检测APS浓度分别为25mg／L、50mg／L、100mg／L、200mg／L、300mg／L时对小鼠BMSC增殖的影响；流式细胞仪检测APS浓度分别为25mg／L、50mg／L、100mg／L、200mg／L、300mg／L时对小鼠BMSC细胞周期和细胞凋亡的影响。结果APS作用后的BMSC达80％贴壁所需时间比对照组明显缩短（P〈0．05）；在同一作用时间下，各APS浓度组BMSC的增殖能力较对照组增强（P〈0．05），其中50mg／L组和100mg／L组中BMSC增殖能力较其他各组明显增强（P〈0．05）；各药物组小鼠BMSC3．5d后各组S期和S＋G2／M期均较对照组明显增多（P〈0．05）；而G0／G1期细胞均较对照组减少（P〈0．05）；50mg／L组和100mg／L组BMSCS期细胞较其它药物组S期细胞数明显增多（P〈0．05）；除50mg／L组外，其余各药物组G2／M期细胞较对照组增多（P〈0．05）。各药物组BMSC的凋亡较对照组降低（P〈0．05），并且浓度为100mg／L时其凋亡较其它各组明显减少（P〈0．05）。结论APS可改变BMSC细胞周期，减少凋亡细胞数目进而促进BMSC的增殖。

Effects of Angelica Polysaccharides on the Proliferation,Cell Cycle and Apoptosis of Mice Bone Marrow Stromal Cell

Objective To explore the effect of Angelica polysaccharides(APS) on the proliferation,cell cycle and apoptosis of mice bone marrow stromal cell(BMSC).Methods The BMSC was cultivated in vitro and randomly devided into control group and drug groups.The time of 80% adherent BMSC seen was checked with different action time in which the concentrations of APS were 50 mg/L and 100 mg/L;MTT was employed to detect the BMSC's proliferation in which the concentrations of APS were 25 mg/L,50 mg/L,100 mg/L,200 mg/L and ...