实验性兔动脉损伤后血管外膜成纤维细胞的变化

目的探索动脉损伤后,血管外膜成纤维细胞的变化。方法以球囊导管法制模,通过病理形态学、免疫组织化学、计算机图像分析和透射电镜观察兔髂动脉损伤后各时间点血管外膜成纤维细胞的增殖与表型转化情况;并通过V染色血管外膜成纤维细胞的分布、测量面积并反映成纤维细胞表型转化后的合成与分泌功能。结果以球囊导管法制造兔髂动脉损伤动物模型100%成功,观察到损伤后外膜细胞首先发生增殖,成纤维细胞发生表型转化,并在电镜下观察到成纤维细胞的迁移现象。结论动脉损伤后,血管外膜成纤维细胞首先增殖,转化为肌成纤维细胞,表达α-肌动蛋白(α-actin),并向内膜转移。

The experimental study on the aIteration of fibroblast in external coat of vessels after rabbjt anery injury

Objective To explore the alteration of fibroilast in external coat of vessels after atery injury. Methods The animal modl was established by Foley,s tube method.The proliferation and phenotype transformation were detected by pathomorphology,immunohisto chemistry cand transmission elextron microscope.The distribution of fibroblst in external cout af ressels was obserured through V stain.Image anabysis was cued to eraluate the synthesis and secretion funtion of fibroblast apter phenotype trans formation.Resulls The animal model was sueessfully established.After artery injury,the fibroblast in external coat of vessets begn to prolifere first,followed by phenotype transformation.The migration of the fibroblast was obseved under the transmission elextron microscope.Conclusion After artery injury,the fibroblast in external coat of vesselts began to proliferate and then transformate to myofibroblast(express a-actin protein) and traslocated into the neointirna.