IL—4基因转染人胃癌细胞的实验研究

目的：评价ＩＬ－４基因转染人胃癌细胞株分泌ＩＬ－４的可行性。方法：用脂质体转染法将ＩＬ－４基因转入ＳＧＣ－７９０１人胃癌细胞株，并检测细胞分泌的ＩＬ－４和对ＬＡＫ细胞杀伤肿瘤细胞作用的影响。结果：ＩＬ－４基因可以转染入人胃癌细胞，并表达有活性ＩＬ－４，基因转染可以明显增强ＩＬ－２激活的ＬＡＫ细胞的肿瘤杀伤作用，用大剂量的γ射线照射后，转染细胞仍可以持续分泌ＩＬ－４３～４周期。结论：ＩＬ－４基因转染不改变胃癌细胞的体外生长特性，但可以明显增强抗肿瘤免疫反应

Enhancement of Host Anti tumor Immunity by Transfecting Human Gastric Carcinoma Cell Line with IL 4 Gene et al

Objective: To assess the possibility of continuous production of IL 4 by a cytokine transfected human gastric carcinoma cell line (GCC).Methods :Plasmid vector was used to transfect the SGC 7901 line with the gene for human IL 4 by lipofectin reagent. Biologic characterisitics of the cytokine transfected tumor cells were determined by MTT co lorimetry and cytotoxicity, fluorescense activated cell sorter analysis and Southern blot. Results: The productive insertion of IL 4 gene was successful. The amount of IL 4 per 10 6 cells per day was 20.0 60.12ng/ml .Morphology, growth rates and MHC I.II antigen expression were identical to those of the parental GCC line. IL 4 producing cells demonstrated increased vulnerability to cell killing of LAK cells. Conclussion: This study provides evidences that IL 4 gene can be transfected into GCC without modifying their phenotype and that this transduction leads to an increased effect on the anti tumor result.