保尔佳对黑色素瘤细胞转移能力及相关细胞行为的影响

考察保尔佳对恶性肿瘤转移的影响及其相关机制。采用细胞微管吸吮实验技术单细胞定量观察不同浓度的保尔佳处理小鼠黑色素瘤细胞不同时间后，瘤细胞对培养牛肺动脉内皮细胞粘附性的改变，同时采用Ｂｏｙｄｅｎ小室法检测经同样处理的瘤细胞的侵袭穿膜能力。采用瘤细胞注射法制作肺转移荷瘤小鼠模型，观察经处理瘤细胞肺转移结节形成数目的改变。保尔佳处理可明显降低瘤细胞与内皮细胞的粘附性，同时抑制其侵袭穿膜能力，这种作用在１０－２～１０μｇ／ｍｌ浓度范围呈剂量依赖性，在１２～４８ｈ范围呈时间依赖性，短时间（３０ｍｉｎ）处理无明显作用。经保尔佳处理的癌细胞，其在肺组织中形成转移结节的数目明显减少。保尔佳通过抑制瘤细胞转移相关性细胞行为—粘附、侵袭，从而起抑制肿瘤转移的作用

The Effect of Polyerga on Cellular Metastatic Potential and Cellular Behavior of Malignant Melanoma

To study the influence of Polyerga on neoplastic metastasis and related cytologic behavior.The adhesion of murine melanoma cells B16 treated by Polyerga of different concentrations for various time to bovine pulmonary edothelial cell (EC) was examined by the use of micromanipulation technique.The invasive ability of the tumor cells prepared in the same manner was measured by the polycarbonate filter of Boyden chamber.The number of metastatic pulmonary nodules of the murine model was observed.Polyerga significantly decreased the adhesiveness of tumor cells to the pulmonary endothelial cells and inhibited the invasiveness of the tumor cells.After the treatment of Polyerga,the number of metastatic nodules in the pulmonary tissue decreased remarkably.The action of suppressing tumor metastasis of Polyerga is accomplished through inhibiting the metastatic ability of cellular behavior,cell adhesion and invasion.