| HPLC检测人血浆中厄贝沙坦的浓度 |
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| 引用本文： | 陈慧敏,赵建波,邱相君,吴明伍,陈汇. HPLC检测人血浆中厄贝沙坦的浓度[J]. 中国现代应用药学, 2010, 27(5): 422-425 |
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| 作者姓名： | 陈慧敏 赵建波 邱相君 吴明伍 陈汇 |
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| 作者单位： | 浙江省中医院保健科;杭州师范大学基础医学部药理学教研室;河南科技大学医学院;华中科技大学同济医学院临床药理研究所; |
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| 摘 要： | 目的建立人血浆厄贝沙坦检测的高效液相色谱法。方法血浆经乙醚萃取,以ZORBAX Extend-C18为色谱柱;流动相为乙腈-水-0.1%TFA-0.5 mmol.L^-1 SDS体系,流速为0.8 mL.min^-1;检测波长为242 nm（0-6.5 min）和266 nm（6.5-7.8min）。结果厄贝沙坦浓度在0.05-6.00 mg.L^-1内线性关系良好（r=0.999 9）;定量限为0.05 mg.L^-1;高中低3个浓度的相对回收率分别为（103.93±1.09）%、（99.85±0.65）%和（100.95±1.21）%;日内RSD分别为3.04%,1.15%,1.19%,日间RSD分别为4.50%,3.32%,1.18%。结论本方法准确可靠、简便快速,适用于人血浆厄贝沙坦浓度的测定及其药动学研究。
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| 关 键 词： | 高效液相色谱法 厄贝沙坦 血药浓度 药动学 |
Determination of Irbesartan in Human Plasma by HPLC |
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| CHEN Huimin,ZHAO Jianbo,QIU Xiangjun,WU Mingwu,CHEN Hui. Determination of Irbesartan in Human Plasma by HPLC[J]. The Chinese Journal of Modern Applied Pharmacy, 2010, 27(5): 422-425 |
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| Authors: | CHEN Huimin ZHAO Jianbo QIU Xiangjun WU Mingwu CHEN Hui |
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| Affiliation: | CHEN Huimin,ZHAO Jianbo,QIU Xiangjun,WU Mingwu,CHEN Hui(.Department of Health Care,Zhejiang Hospital of Chinese Medicine,Hangzhou,Chin,.Department of Pharmacology,Hangzhou Normal University,Hangzhou,.Medical College of Henan University of Science,Technology,Luoyang,.Institute of Clinical Pharmacology,Tongji Medical College of Huazhong University of Science,Technology,Wuhan,China |
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| Abstract: | OBJECTIVE To develop a high performance liquid chromatography method for the determination of irbesartan in human plasma.METHODS The plasma were extracted by diethyl ether.The analytical column was packed with ZORBAX Extend-C18.The mobile phase were acetonitrile-water-0.1% trifluoroacetic acid-0.5 mmol.L-1 SDS and the flow rate was 0.8 mL.min-1.The UV detection wavelength was 242 nm(0-6.5min) and 266 nm(6.5-7.8min).RESULTS Excellent liner relationship was obtained in the range of 0.05-6.00 mg.L-1(r=0.999 9)... |
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| Keywords: | HPLC irbesartan plasma concentration pharmacokinetics |
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