MMC—1类巨噬细胞吞噬作用的透射电镜观察

OBSERVATION OF PHAGOCYTOSIS OF MMC-1 MACROPHAGF-LIKE CELL LINE WITH TRANSMISSION ELECTRON MICROSCOPE

The phagocytosis of opsonic chicken erythrocytes which were engulfed by MMC-1 cells was studied by means of transmission electron microscopy.After incu- bating chicken erythrocytes with the serum of rabbit-anti chicken antibody.MMC-1 cells were surrounded by 1 to more than 10 opsonized erythrocytes.The EA rosettes were formed.At first,opsonized erythrocytes attached to the MMC-1 cells in some points.At the site of interaction of opsonized erythrocytes with MMC-1 cells the electron dense bands which were the specialization of the MMC-1 cell plasma mem- brane appeared.Then many projections of the erythrocytes were engulfed into MMC-1 cells.When part of the erythrocyte was engulfed by macrophage,a collar- like structure arround the erythrocyte could be seen.Partially engulfed erythrocytes looked like a dumbbell.The engulfed erythrocytes were irregular in shape.The density of engulfed erythrocytes became faint gradually.The round ring with high electron density appeared and enlarged in erythrocytes.It was shown that the opso- nized erythrocytes which formed EA rosettes were engulfed by MMC-1 phagocyte. After engulfing many erythrocytes,MMC-1 cells might degenerated or be engulfed by other MMC-1 cells.It was proved that Fc receptors presented on MMC-1 cells. Many mitochondria were easily found in MMC-1 cells,they were responsible for energy supplies.Extruding of engulfed nuclei from macrophage could be seen too. It was shown that MMC-1 cells had the characteristics of macrophage and it was established by cell fusion of phagocyte and thymoma cell.