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Ultracryotomy: development and application (with examples from the yeast cytology) (author's transl) |
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| Authors: | Konrad Joachim Böhm |
| Affiliation: | Akademie der Wissenschaften der DDR, Forschungszentrum für Molekularbiologie und Medizin, Zentralinstitut für Mikrobiologie und Experimentelle Therapie, Jena (Direktor: Prof. Dr. U. Taubeneck) |
| Abstract: | The preparation steps usually necessary for obtaining ultrathin frozen sections of biological material (chemical prefixation, enclosing, cryoprotective treatment, freezing, sectioning, and post-staining the sections for transmission electron microscopy) are submitted to a critical analysis. The application of cryo-ultramicrotomy, in particularly for cytochemical purposes, is reviewed. Fundamental considerations of chemical prefixation and poststaining are supported by examples from yeast cytology. Furthermore, the efficiency of the cryo-ultramicrotomy (electron optical resolution of ultrastructural details) is demonstrated on yeast cells and protoplasts. |
| Keywords: | CHR Chromatin CW Zellwand E Einbettungsmedium ER endoplasmatisches Reticulum F Filamentbündel K Proteinkristalle KT Kristall-assoziierte Tubuli M Mitochondrien MT Mikrotubuli der Mitosespindel N Kern NAO Kern-assoziiertes Organell NE Kernhülle NP Kernporen P Plasmamembran R Ribosomen T Tubuli V Vakuole |
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