Dicer表达与肾透明细胞癌发生及转移的关系

目的：探讨Dicer在肾透明细胞癌发生及转移中的作用。方法：选取正常肾小管上皮细胞株HKC、非转移性肾透明细胞癌细胞株769P、转移性肾透明细胞癌细胞株Caki-1以及36例肾透明细胞癌手术标本（其中11例已发生远处转移）和相应癌旁正常肾组织，应用实时定量PCR和Western blot方法检测Dicer在肾透明细胞癌细胞株和组织中mRNA和蛋白的表达情况，并分析Dicer的mRNA水平与临床病理资料的关系。结果：和正常肾小管上皮细胞株HKC相比；Dicer的mRNA水平在肾透明细胞癌细胞株769-P和Caki-1中均降低（P〈0．001），而转移性肾透明细胞癌细胞株Caki-1比非转移性肾透明细胞癌细胞株769-P表达水平更低（P〈0．001）；和癌旁正常肾组织相比，Dicer的mRNA水平在‘肾透明细胞癌手术标本中明显降低（P〈0．001），且已发生远处转移的。肾癌标本比未发生远处转移的肾癌标本表达水平更低（P=0．04）；Dicer在细胞株和组织中的蛋白水平的变化与mRNA水平的变化一致（P〈0．001）；Dicer的mRNA水平在不同年龄、性别、组织学分级、肿瘤大小及T分期组间无统计学差异（P〉0．05）。结论：Dicer表达降低可能在。肾透明细胞癌的肿瘤发生中发挥作用，且其表达的进一步下降可能与肾透明细胞癌的远处转移有关。

Dicer expression in relation to tumorigenesis and metastasis of clear cell renal cell carcinoma

Objective：To explore the role of Dicer in tumorigenesis and metastasis of clear cell renal cell carci-noma （ccRCC）. Method：The expression of Dicer in mRNA and protein levels were detected in human kidney tubule epithelial cell line HKC, non-metastatic ccRCC cell line 769-P, metastatic ccRCC cell line Caki-1, and 36 cases of ccRCC surgical specimens（including 11 cases with distant metastasis）and their corresponding adjacent normal renal tissues by real-time PCR and Western blot respectively, analyzing the relationship between the mRNA levels of Dicer and clinicopathological variables. Result：Compared to human kidney tubule epithelial cell line HKC, the mR- NA levels of Dicer were significantly down regulated in ccRCC cell lines of 769-P and Caki 1 （ P 〈0. 001）, with the metastatic ccRCC cell line Caki-1 even lower（ P 〈0. 001）. Meanwhile, the mRNA levels of Dicer were signifi- cantly down-regulated in ccRCC surgical specimens compared to adjacent normal renal tissues （ P〈0.001）, with the metastatic ones further reduced （ P =0. 04）. The alteration of the protein levels in ccRCC cell lines and tissues were consistent with that of the mRNA levels （ P〈0. 001）. The mRNA levels of Dicer were not significantly dif-ferent among different groups, in terms of age, sex, histological staging, tumor size and T staging （ P〉0.05）.Conclusion： Reduced expression of Dicer may play a role in the tumorigenesis of ccRCC, and expression of a furtherdecline may relate to distant metastasis of ccRCC.