运动神经传导检测神经再生的实验研究

目的：本实验通过观察SD大鼠坐骨神经完全切断再植后维生素对神经生长的影响,运用神经传导检测并统计分析。方法：20只完全切断坐骨神经再植大鼠随机分成维生素组和空白对照组（10只/组）。在6周、10周和14周时行肌电图仪电刺激大鼠的坐骨神经远、近端,在腓肠肌肌腹部记录复合肌肉动作电位并统计分析。同时进行超强刺激,观察有无M波出现,并与健侧对照。结果：SD大鼠坐骨神经再植后轴突从6周左右就已经进入远端,肌肉容积开始恢复。神经传导检测表明出现相对完整的运动神经传导波形。10周时超强刺激检测维生素组已经有2只出现M波;14周时超强刺激M波的检测结果卡方检验差异有统计学意义。但是,14周时大鼠仍不能够自主张开脚趾行走。结论：运动神经传导检测神经再生的意义主要表现在波幅上更为客观,而神经传导速度和潜伏期误差较大。在10周时超强刺激就出现M波,M波的出现率有助于判断神经再生。

Study on Regeneration Sciatic Nerve of Rats Detected by Motor Nerve Conduction Velocity

Objective： To observe the impaction of vitamins on the nerve growth by motor nerve conduction velocity after sciatic totally cut off.Methods： All of twenty SD rats were randomly divided into control group and vitamin therapy group.Electric stimuli were performed to the distal and proximal sciatic nerve trunks and analyzed at the sixth、tenth and fourteenth week.Whether the M wave appeared was observed,when the super-stimulation were performed.Results： Axons have entered the remote of nerve from the 6th week after anastomosed graft of nerve,and muscle volume has begun to recover.The CMAP at gastrocnemius muscle belly was detected,after operation 6 weeks.The M-wave was detected in two rats in vitamin therapy group.Although,there was significant difference in both scores of M-wave at 14th week postoperatively,the rats still could not move.Conclusion： Vitamin B1and vitamin B12 can promote growth of nerve h and hasten the growth of α nerve fibers after implanted 6-10 weeks.