外周血单个核细胞中乙型肝炎病毒前S／S基因变异？…

目的 研究外周血单个核细胞（ＰＢＭＣ）中乙型肝炎病毒（ＨＢＶ）包膜抗原基因变异的意义。方法 收集１９例慢性乙肝患者的ＰＢＭＣ和配对血清，用ＰＣＲ扩增ＰＢＭＣ及血清中ＨＢＶ包膜基因片段９Ｓ，ｐｒｅＳ１，ｐｒｅＳ２），并对其中各５例ＰＢＭＣ及配对血清中的ｐｒｅＳ１和ｐｒｅＳ２基因片段，分别进行核苷酸序列分析。结果 Ｓ，ｐｒｅＳ１和ｐｒｅＳ２片段在血清和ＰＢＭＣ中的检出率，分别为１００％，９４．＆％，１

Study on HBV preS/S gene mutation in peripheral blood mononuclear cells]

OBJECTIVE: To study the significance of mutations of HBV preS and S genes in peripheral blood mononuclear cells (PBMC). METHODS: Srea and PBMCs of 19 chronic hepatitis B patients were collected. The S, preS1 and preS2 gene fragments of HBV were separately amplified by PCR, and 5 cases of preS1 and preS2 gene fragments in paired sera and PBMC were sequenced. RESULTS: The positive rates of S, preS1 and preS2 gene fragments in serum and PBMC were 100%, 94.7%, 100% and 0, 26.3%, 26.3% respectively. Sequence analysis of preS1 and preS2 genes showed that in 8/10 specimens there were point mutations in preS1 and 7/10 mutations in preS2; while in the others (2 of preS1 and 3 of preS2) the sequences were identical to that of wild-type HBV. CONCLUSION: No full-length HBV genome in PBMC was detected by PCR, this is a fact not supporting that HBV can replicate, assemble and release from PBMC. The amino acid changes predicted mutatieds from nucleotide were focused in aa21-47 region, which might affect adsorption and penetration of HBV, and alteration in tissue tropism. Whether the presence of HBV subgenomic fragments in PBMC can affect cell function should be pursued.