线探针核酸杂交分析贵州地区HCV感染株基因型

目的　分析贵州地区丙型肝炎病毒（ＨＣＶ）感染株的基因型。方法　用第二代线探针核酸杂交方法，对９８ 例ＨＣＶ感染者血清进行基因分型。结果　１２ 例献血员血清，均为ＨＣＶ１ｂ 型；１５ 例血液病患者中，１４ 例（９３－３％ ）为ＨＣＶ１ｂ ，１ 例（６－７％ ）为ＨＣＶ１ｂ ＋ ２ 型。慢性丙肝患者３７ 例中，ＨＣＶ１ｂ 型３４例（９１－９％ ） ，混合感染基因型３ 例（ＨＣＶ１ａ ＋ １ｂ 、ＨＣＶ１ｂ ＋ ２ 、ＨＣＶ１ａ ．１ｂ ＋ ２ａ２ｃ 各１ 例）。静脉药瘾者３４ 例，其中ＨＣＶ１ｂ 感染１３ 例（３８－２％ ） ，ＨＣＶ６ａ１０ 例（２９－４％ ） ，ＨＣＶ３ｂ４ 例（１１－８％ ） ，ＨＣＶ３ａ１ 例（２－９ ％） ，混合感染６ 例（１７－６ ％） ，大多为ＨＣＶ２ 复合其他基因型。结论　贵州地区ＨＣＶ感染者中，以ＨＣＶ１ｂ 基因型为主，其次为ＨＣＶ６ａ ，尚存在ＨＣＶ３ａ 和ＨＣＶ３ｂ 。其中ＨＣＶ３ａ、３ｂ 、６ａ ，在本地区系首次报告，但主要存在于静脉药瘾者中。虽然有混合感染基因型的存在，但其基因基础需测序分析

Analysis of hepatitis C virus genotypes in Guizhou area, using second generation line probe assay

OBJECTIVE: To analyse the frequency of various hepatitis C virus genotypes in different populations with HCV infection from Guizhou area. METHODS: Ninty-eight HCV isolates obtained by using RT-nested PCR from 37 patients with chronic hepatitis C (PHCs), 15 patients with hemopathy (PHs), 12 blood donors (BDs) and 34 intravenous drug-users (IVDUs) were genotyped by using the second generation line probe assay. RESULTS: Among 37 HCV isolates from PHCs, there were 34 (91.9%) genotype 1b, 3 (8.1%) mixted genotypes (1 for 1a + 1b, 1 for 1b + 2, 1 for 1a + 1b + 2a/2c, respectively). Among 15 PHs, 14 (93.3%) were type 1b, 1(6.7%) were type 1b + 2, among 12 BDs, all(100.0%) were type 1b. Among 34 IVDUs, 13(38.2%) were type 1b, 10(29.4%) were type 6a, 1(2.9%) was type 3a, 4(11.8%) were type 3b and 6(17.6%) were mixed types(2 for 1a + 2, 1 for 1a, 1b + 2, 1 for 1b + 3b, 1 for 3b + 2, 1 for 1b + 6a, respectively). CONCLUSION: The results showed that HCV subtypes 1a, 1b, 2a/2c, 3a, 3b and 6a belonging to 4 HCV genotypes(1,2,3,6) were found from different populations in Guizhou, of these, 3a, 3b and 6a were only found in IVDUs and their total frequency was above 50%.