| 检测HBV基因组nt585位突变的特异性聚合酶链反应 |
| |
| 引用本文： | 房德兴,李法卿,谭维国.检测HBV基因组nt585位突变的特异性聚合酶链反应[J].中华实验和临床病毒学杂志,1999,13(4):373-376. |
| |
| 作者姓名： | 房德兴 李法卿 谭维国 |
| |
| 作者单位： | 南京军区军事医学研究所(房德兴!210002南京，李法卿!210002南京，谭维国!210002南京，王元伦!210002南京，陈华标!210002南京)，南京第二医院(林厚基，周镇先) |
| |
| 基金项目： | 江苏省自然科学基金!编号BK97188 |
| |
| 摘 要： | 目的 为调查乙型肝炎病毒(HBV)nt585位A→C变异株在国内的流行情况提供方法学。方法 根据已知的HBV基因组序列并结合国内主要流行adr和adw亚型的特点,合成在nt585位分别为A和C的2套4对引物,建立了突变特异性聚合酶链反应(msPCR)方法。结果 利用msPCR对由25份乙型肝炎免疫失败儿童患者血清和32份成人乙型肝炎患者血清扩增的HBVS基因片段进行了初步检测。结果表明该法可特异性扩增nt585为A和C的HBVDNA。结论 该法是鉴定HBV基因组nt585位A→C变异株的特异而敏感的方法。
|
| 关 键 词： | 肝炎病毒 乙型 突变 聚合酶链反应 |
A mutation specific polymerase chain reaction for detecting hepatitis B virus genome with A-to-C mutation at nt585 |
| |
| FANG Dexing,LI Faqing,TAN Weiguo,et al..A mutation specific polymerase chain reaction for detecting hepatitis B virus genome with A-to-C mutation at nt585[J].Chinese Journal of Experimental and Clinical Virology,1999,13(4):373-376. |
| |
| Authors: | FANG Dexing LI Faqing TAN Weiguo |
| |
| Institution: | Huadong Research Institute for Medical Biotechnics, Nanjing 210002. |
| |
| Abstract: | OBJECTIVE: To investigate the prevalence of hepatitis B virus (HBV) nt585 A-to-C mutants in China. METHODS: A mutation specific polymerase chain reaction (msPCR) was established for amplifying HBV DNAs with A-to-C mutation at nt585. Two sets of primer pairs with same sequences except for one base at 3' terminus were designed and synthesized according to 48 of the known HBV genome sequences and the popular HBV subtypes, adr and adw, in China. RESULTS: 57 of HBV S gene fragments amplified by a nested PCR from different and child adult hepatitis B patients were characterized by using the msPCR. It was shown that HBV mutants with A-to-C mutation at nt585 could be confirmed. CONCLUSION: The msPCR is a specific and sensitive method for detecting the HBV nt585 A-to-C mutants. |
| |
| Keywords: | Hepatitis B virus Mutation Polymerase chain reaction |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
