宫颈癌组织中人乳头瘤病毒16型L1基因的克隆及序列分析

目的人乳头瘤病毒１６型（ＨＰＶ１６）晚期基因区（Ｌ１）编码病毒的主要衣壳蛋白，且ＨＰＶ１６感染与宫颈癌发生、发展关系密切，故对中国妇女感染的ＨＰＶ１６基因进行克隆及序列分析有重要实际意义。方法采用聚合酶链反应技术（ＰＣＲ）扩增了３例中国妇女宫颈癌组织中ＨＰＶ１６的Ｌ１基因片段，并对其进行了克隆及全序列分析。结果发现３个ＨＰＶ１６Ｌ１基因核苷酸序列有４处均与最初报道的ＨＰＶ１６Ｌ１ＤＮＡ序列不同，并由此引起所编码的氨基酸亦发生变化。结论中国妇女宫颈癌组织中ＨＰＶ１６Ｌ１核苷酸有一定程度变异。

Cloning and sequencing of human papillomavirus 16 L1 gene from cervical carcinoma tissues of Chinese women

Objective Human papillimavirus type 16 (HPV16) is highly related with the development of cervical carcinoma. HPV16 late gene L1 encodes its main capsid protein. This study is to analyze the whole sequence of L1 gene of HPV16 of the Chinese isolates. Methods Three samples of HPV16 L1 gene were amplified from cervical carcinoma tissues of Chinese patients by PCR and then cloned and sequenced. Results There were four sites in nucleic acid sequences of all three HPV16 L1 fragments were different from the originally reported sequence of HPV16 and the differed sequences had changed the triplet codes, therefore, subsequenely changed the amino acids it coded. Conclusion The results showed that some mutation had taken place in the nucleotide sequence of L1 gene of HPV16 obtained from the cervical carcinoma tissues of Chinese women.