人喉癌组织中人乳头瘤病毒DNA的检测

目的为探讨喉癌与人乳头瘤病毒（ＨＰＶ）感染的关系和ＨＰＶ在喉癌中基因组型的分布与表达。方法应用聚合酶链反应技术（ＰＣＲ）制备非放射性探针标记物－地高辛标记ＨＰＶ共有引物探针，对１４６例喉不同病变的新鲜组织标本（喉癌６８例，喉其它病变４８例，正常喉组织３０例），进行ＨＰＶ６，１１，１６，１８，３１，３３，３５，４２，５８共９型ＨＰＶＤＮＡ感染的检测；阳性者用多重引物ＰＣＲ方法分型。结果喉癌ＨＰＶ感染阳性率４５．６％（３１／６８），喉癌颈转移淋巴结组织阳性率２０．０％（３／１５），喉癌前病变阳性率１１．８％（２／１７），声带息肉阳性率６．３％（１／１６），１５例癌旁及１５例癌周正常喉组织均为ＨＰＶＤＮＡ阴性。ＨＰＶＤＮＡ型别分布在喉癌中以ＨＰＶ１６、１８型为主，喉良性病变中以ＨＰＶ６、１１型为主。结论喉癌发生与ＨＰＶ感染有关。

Detection of human papillomavirus in laryngeal carcinoma with digoxigenin labelled probe prepared by polymerase chain reaction]

OBJECTIVE: To study the relationship between laryngeal carcinoma and human papillomavirus (HPV), and describe distribution, expression of genome types of HPV in laryngeal carcinoma. METHODS: We used a non-radioactive digoxigenin labeled probe prepared by polymerase chain reaction (PCR) to test the fresh tissue samples from 146 cases with different laryngeal lesions. The labelled probe of HPV consensus primers was obtained, which enabled detection of nine types of HPV DNAs that were HPV 6, 11, 16, 18, 31, 33, 35, 42 and 58. The genome types of HPV DNA positive cases were determined by using multiple primers PCR. RESULTS: The results turned out to be as follows: The HPV infection positive rate was 45.6%(31/68) for laryngeal carcinoma, 20.0% (3/15) for neck metastatic lymphnode, 11.8%(2/17) for precarcinomatous lesions and 6.3%(1/16) for vocal cord polypus. 15 cases of normal tissues adjacent to carcinoma and another 15 normal tissues opposite to the carcinoma were all HPV DNA negative. HPV16, 18 types are the main types of laryngeal carcinoma and HPV6, 11 types often appear in benign lesions of larynx. CONCLUSION: These results implicated that the occurrence and development of laryngeal carcinoma may be closely related to HPV infection.