沈阳地区各型丙型肝炎患者丙型肝炎病毒的基因分型

目的研究沈阳地区丙型肝炎病毒（ＨＣＶ）基因型分布状况及其临床意义。方法应用聚合酶链反应（ＰＣＲ）方法对沈阳地区８４例血清抗－ＨＣＶ及ＨＣＶＲＮＡ均阳性的各临床型丙型肝炎患者进行ＨＣＶ基因分型。结果８４例丙型肝炎中，ＨＣＶ－Ⅱ型４５例（５３．６％），ＨＣＶ－Ⅲ型２６例（３０．９％），Ⅱ／Ⅲ混合型１３例（１５．５％）；ＨＣＶ不同基因型在各型丙型肝炎中总体分布不一致，经统计学处理，Ｐ＜０．０５；在急性肝炎、慢性肝炎轻度、中度、重度及肝炎后肝硬化中，ＨＣＶ－Ⅱ型感染分别占５５．６％，３６．２％，７５．０％，８５．７％及８８．９％；ＨＣＶ－Ⅲ型感染分别占２２．２％，４６．８％，８．３％，０％骸１１．１％。结论沈阳地区ＨＣＶ感染以Ⅱ型为主，其次为Ⅲ型及Ⅱ／Ⅲ混合型；ＨＣＶ基因型与丙型肝炎严重程度密切相关。

Study on HCV genotypes in different clinical types of hepatitis C patients in Shenyang area

OBJECTIVE: To study the distribution of HCV genotypes in different clinical types of hepatitis C in Shenyang area and its clinical significance. METHODS: The HCV genotypes of 84 HC patients of different clinical types were detected by PCR assay with four kinds of type-specific primers. RESULTS: In 84 patients with hepatitis C, the HCV-II and HCV-III genotypes and the II/III mixed genotype were 53.6%, 30.9% and 15.5% respectively. The distribution of HCV genotypes in different HC clinical types was not identical, P < 0.05. In acute hepatitis, in mild, moderate and severe degree of chronic hepatitis, and in liver cirrhosis, the infection rates of genotype II HCV were 55.6%, 36.2%, 75.0%, 85.7% and 88.9% respectively, but that of genotype III were 22.2%, 46.8%, 8.3%, 0% and 11.1% respectively. CONCLUSION: Infection of geno type II HCV was predominant in Shenyang area followed by HCV-III and II/III mixed genotypes. The different genotypes of HCV were related to the severity of hepatitis C.