| 刺参黏多糖对Hela细胞PCNA表达及细胞周期的影响 |
| |
| 引用本文： | 张笑雪,于壮,宋扬.刺参黏多糖对Hela细胞PCNA表达及细胞周期的影响[J].山东医药,2008,48(46):19-21. |
| |
| 作者姓名： | 张笑雪 于壮 宋扬 |
| |
| 作者单位： | 1. 青岛大学医学院附属医院,山东青岛,266003
2. 青岛大学营养学研究室 |
| |
| 基金项目： | 山东省科技厅资助项目(2005GG3205059) |
| |
| 摘 要： | 目的研究刺参黏多糖（SJAMP）对体外培养的Hela细胞增殖细胞核抗原（PCNA）表达及细胞周期的影响。方法用免疫组织化学法测定SJAMP对体外培养的Hela细胞作用后的PCNA表达水平，用流式细胞术测定细胞周期变化，并与甲氨蝶呤（MTX）比较。结果SJAMP、MTX均可使体外培养的Hela细胞PCNA表达水平明显降低，还可以使细胞周期发生明显的变化，干预组Hela细胞停留在G．期的比例要比未干预组明显增加（P〈0．05），而S、G2期的细胞比例比未干预组则明显减少（P〈0．05）。结论SJAMP有下调Hela细胞PCNA表达水平，阻滞细胞周期的作用。
|
| 关 键 词： | 刺参黏多糖 甲氨蝶呤 Hela细胞 细胞增殖 细胞周期 |
Effects of stichopus japonicus acidic mucopolysaccharide on PCNA expression and cell cycling in henrietta lacks strain of cancer cells |
| |
| ZHANG Xiao-xue,YU Zhuang,SONG Yang.Effects of stichopus japonicus acidic mucopolysaccharide on PCNA expression and cell cycling in henrietta lacks strain of cancer cells[J].Shandong Medical Journal,2008,48(46):19-21. |
| |
| Authors: | ZHANG Xiao-xue YU Zhuang SONG Yang |
| |
| Institution: | 1 Affiliated Hospital of Medical College of Qingdao University;Qingdao266003;P.R.China |
| |
| Abstract: | Objective To investigate the effects of stichopus japonicas acidic mucopolysaccharide(SJAMP) on PCNA expression and cell cycling in invitro cultured henrietta lacks strain of cancer cells(Hela cells) and compare these with methotrexate(MTX).Methods The cell cycle was measured using flow cytometry(FCM),and the level of proliferating cell nuclear antigen(PCNA) with immunohistochemistry(IHC).The results were compared between SJAMP and MTX.Results Both SJAMP and MTX led to significantly suppressed expression of... |
| |
| Keywords: | stichopus japonicus acidic mucopolysaccharide methotrexate henrietta lacks strain of cancer cells cell proliferation cell cycle |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
