人重组粒细胞集落刺激因子动员后采集骨髓对健康供者外周血采集物成份的影响

本研究探讨健康供者体内应用人重组粒细胞集落刺激因子（G—CSF）后采集骨髓对外周血采集物成份的影响。62例健康供者皮下注射rhG—CSF5μg/（kg·d），连用5天，其中31例供者在第4、5天分别采集骨髓和外周血采集物（A组）；另31例供者于第4、5天均单采集外周血单个核细胞（B组）。应用流式细胞术检测两组供者外周血采集物中的淋巴细胞、CD3^＋、CD3^＋CD4^＋、CD3^＋CD8^＋、CD14^＋、CD34^＋细胞以及CD3^＋CD4^-CD8^-T细胞的数量。结果显示，A组供者每微升外周血采集物中单个核细胞中CD3^＋、CD3^＋CD4^＋、CD3^＋CD8^＋、CD14^＋、CD34^＋细胞以及CD3^＋CD4^-CD8^-T细胞的中位含量分别1．56×10^5μ1、8．56×10^4μl、6．12×10^4μl、3．38×10^4μl、2．27×10^4μl、3．83×10^4μl、744μl及3588μl，与B组的1．40×10^5μl、7．34×10^4μ1、5．32×10^4l、3．06×10^4μl、1．83×10^4μl、3．21×10^4μl、554μl及3120μl的差异无统计学意义（p〉0．05）；A组外周血采集物中CD4^＋细胞与CD8^＋细胞的比值1．52（0．54—2．87）]、单核细胞与CD3^＋细胞的比值0．57（0．15—1．64）]以及CD3^＋CD4^-CD8^-T细胞与CD3^＋细胞0．064（0．018—0．673）]的比值与B组1．68（0．31—3．35）]、0．59（0．18—1．25）]、0．063（0．021—0．136）]的差异均无统计学意义（P〉0．05）。结论：健康供者体内应用rhG—CSF后采集骨髓对外周血采集物成份无影响，对rhG—CSF动员的同一健康供者可单独采集骨髓、外周血采集物或同时采集两种采集物以满足临床移植的需要。

Effect of Bone Marrow Graft Collection on Composition of Peripheral Blood Stem Cell Grafts from Healthy Donors after Recombinant Human Granulo-cyte Colony-Stimulating Factor Application In Vivo for Mobilization

The study was purposed to investigate the effect of bone marrow graft collection on the composition of peripheral blood stem cell grafts in healthy donors after recombinant human granulocyte colony-stimulating factor (rhG-CSF) application in vivo. Sixty-two healthy donors were treated with rhG-CSF 5 microg/(kg.d)] injected subcutaneously for five consecutive days. Donors were divided into groups A and B, and 31 donors were there in each group. Bone marrow grafts and peripheral blood stem cell grafts were harvested on the day 4 and 5 respectively in group A. In group B, only peripheral blood grafts were collected on both the day 4 and 5. The quantities of the cell components, CD3(+), CD3(+)CD4(+), CD3(+)CD8(+), CD14(+), CD34(+) cells and CD3(+)CD4(-)CD8(-) T cells were determined by multi-color flow cytometry. The results showed that median counts of nuclear cells (1.56 x 10(5)), lymphocytes (8.56 x 10(4)), CD3(+) (6.12 x 10(4)), CD3(+)CD4(+) (3.38 x 10(4)), CD3(+)CD8(+) (2.27 x 10(4)), CD14(+) (3.83 x 10(4)), CD34(+) cells (744) and CD3(+)CD4(-)CD8(-) T cells (3588) per microliter of peripheral blood stem cell grafts in group A were similar to counts of nuclear cells (1.40 x 10(4)), lymphocytes (7.34 x 10(4)), CD3(+) (5.32 x 10(4)), CD3(+)CD4(+) (3.06 x 10(+)), CD3(+)CD8(+) (1.83 x 10(4)), CD14(+) (3.21 x 10(4)), CD34(+) cells (554) and CD3(+)CD4(-)CD8(-) T cells (3120) in group B (p > 0.05). There were no difference in the ratios of CD4(+) cells/CD8(+) cells, CD14(+) cells/CD3(+) cells and CD3(+)CD4(-)CD8(-) T cells/CD3(+) cells in peripheral blood stem cell grafts between group A 1.52 (0.54 - 2.87)], 0.57 (0.15 - 1.64)], 0.064 (0.018 - 0.673)] and group B 1.68 (0.31 - 3.35)], 0.59 (0.18 - 1.25)], 0.063 (0.021 - 0.136)] (p > 0.05). It is concluded that no effect of bone marrow graft collection on the composition of peripheral blood stem cell grafts in the same donor is found after rhG-CSF application in vivo, bone marrow grafts and peripheral blood stem cell grafts can be collected respectively or simultaneously.