| 舒尼替尼体内诱导耐药鼻咽癌细胞表达NKG2DLs增强NK细胞的抑瘤作用 |
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| 引用本文： | 黄宇贤,王 杨,李玉华,宋朝阳,陈土珍,周 璇,涂三芳,何颖芝,郭坤元.舒尼替尼体内诱导耐药鼻咽癌细胞表达NKG2DLs增强NK细胞的抑瘤作用[J].中国肿瘤生物治疗杂志,2010,17(3):262-267. |
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| 作者姓名： | 黄宇贤 王 杨 李玉华 宋朝阳 陈土珍 周 璇 涂三芳 何颖芝 郭坤元 |
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| 作者单位： | 1. 南方医科大学,珠江医院,血液科,广东,广州,510282
2. 广东省人民医院,肺癌研究所,广东,广州,510080 |
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| 基金项目： | 国家自然科学基金资助项目（No. 30973454）；广东省自然科学基金重点项目（No. 9251051501000007） |
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| 摘 要： | 目的：探讨体内条件下舒尼替尼对耐药鼻咽癌CNE2/DDP细胞NKG2DLs（natural killer group 2 member D ligands)表达的诱导作用，及其对NK细胞抗肿瘤活性的影响。方法：建立ABCG2highCNE2/DDP和ABCG2lowCNE2/DDP细胞裸鼠皮下移植瘤模型，分如下8组：A、E组分别接种ABCG2high、ABCG2lowCNE2/DDP细胞，B、F组分别接种舒尼替尼处理的ABCG2high、ABCG2lowCNE2/DDP细胞，C、G组分别接种ABCG2high、ABCG2lowCNE2/DDP细胞后再输注NK细胞；D、H组接种舒尼替尼处理的ABCG2high、ABCG2lowCNE2/DDP细胞后再输注NK细胞。检测各组裸鼠成瘤时间、成瘤率、肿瘤体积和抑瘤率。免疫组织化学法检测移植瘤组织中NKG2DLs的表达。结果：A、B、C、D和E、F、G、H组肿瘤出现时间分别为(5.43±1.00)、(8.50±035)、(1110±1.25)、(13.56±1.23) d和 (9.00±1.00)、(12.30±0.78)、(14.50±0.50)、(17.25±0.77) d，其中舒尼替尼与NK细胞联合处理组（D 和H 组）成瘤时间最晚（P<0.01）。A、B、C、D和E、F、G、H组肿瘤质量分别为(2.63±089)、(1.00±003)、(065±0.08)、(0.21±0.27) g和(2.79±0.83)、(1.18±0.77)、(0.96±0.50)、(0.86±0.82) g，其中舒尼替尼与NK细胞联合处理组肿瘤质量最小（P<001）；舒尼替尼与NK细胞联合处理的D组和H组的抑瘤率分别为92%和69%。舒尼替尼上调移植瘤组织中NKG2DLs的表达，且ABCG2highCNE2/DDP细胞移植瘤中的NKG2DLs表达率高于ABCG2lowCNE2/DDP细胞。结论：舒尼替尼可在体内诱导CNE2/DDP移植瘤组织表达NKG2DLs，增强NK细胞的抗肿瘤作用。
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| 关 键 词： | 舒尼替尼 自然杀伤细胞 NKG2D配体 鼻咽肿瘤 裸鼠移植瘤 |
| 收稿时间： | 2009/12/12 0:00:00 |
| 修稿时间： | 2010/1/28 0:00:00 |
Sunitinib enhances inhibitory effect of NK cells against xenografts in nude mice by up regulating NKG2DLs expressions in multidrug resistant nasopharyngeal carcinoma cells |
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| HUANG Yu-xian,WANG Yang,LI Yu-hu,SONG Chao-yang,CHEN Tu-zhen,ZHOU Xuan,TU San-fang,HE Ying-zhi and GUO Kun-yuan.Sunitinib enhances inhibitory effect of NK cells against xenografts in nude mice by up regulating NKG2DLs expressions in multidrug resistant nasopharyngeal carcinoma cells[J].Chinese Journal of Cancer Biotherapy,2010,17(3):262-267. |
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| Authors: | HUANG Yu-xian WANG Yang LI Yu-hu SONG Chao-yang CHEN Tu-zhen ZHOU Xuan TU San-fang HE Ying-zhi and GUO Kun-yuan |
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| Institution: | Department of Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong, China;Lung Cancer Institute, Guangdong General Hospital, Guangzhou 510080, Guangdong, China;Department of Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong, China;Department of Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong, China;Department of Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong, China;Department of Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong, China;Department of Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong, China;Department of Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong, China;Department of Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong, China |
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| Abstract: | Objective To investigate the effect of sunitinib on NKG2D ligands(NKG2DLs) expressions and its influences on anti-tumor effect of NK cells.Methods: ABCG2highCNE2/DDP-and ABCG2lowCNE2/DDP cell-implanted mouse tumor models were established and were divided into the following 8 groups.A,E: inoculated with ABCG2highCNE2/DDP or ABCG2low CNE2/DDP cells;B,F: inoculated with sunitinib-stimulated ABCG2highCNE2/DDP cells or ABCG2lowCNE2/DDP cells;C,G: inoculated with ABCG2highCNE2/DDP cells or ABCG2lowCNE2/DDP cells ... |
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| Keywords: | sunitinib natural killer cell natural killer group 2 member D ligand(NKG2DL) nasopharyngeal neoplasmas xenograft in nude mice |
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