ANA、抗SSA抗体、抗SSB抗体阳性患者血细胞分析参数的变化

目的探讨抗核抗体(ANA)、抗干燥综合征A抗体(抗SSA抗体)、抗干燥综合征B抗体(抗SSB抗体)均阳性患者血细胞分析参数的变化。方法采用间接免疫荧光法(IIF)检测ANA,采用免疫印迹法检测抗SSB抗体和抗SSA抗体,以日本希森美康XE-2100全自动仪检测血红蛋白(Hb)、白细胞(WBC)、血小板(PLT)、中性粒细胞百分比(NE%)、淋巴细胞百分比(LY%)。结果与ANA荧光、抗SSA抗体、抗SSB抗体均阴性对照组相比,ANA、抗SSA抗体、抗SSB抗体均阳性组中,血细胞分析参数Hb、WBC、PLT明显减少,差异有统计学意义(P<0.05),其中仅Hb减少53例占29.94%;仅WBC减少59例占33.33%;仅PLT减少24例占13.56%;Hb和WBC同时减少46例占10.50%;WBC和PLT同时减少12例占6.78%,Hb和PLT同时减少26例占14.59%;Hb、WBC、PLT三者同时减少12例占6.78%。结论血液系统自身抗体阳性患者的异常损伤造成的血细胞分析参数变化对临床自身免疫病的诊断治疗具有一定意义。

Analysis of Blood cell parameters in patients with positive ANA,anti-SSA antibody and anti-SSB antibody

Objective To explore the blood cell parameters changing of patients with positive antinuclear antibody(ANA),anti-SSA antibody and anti-SBS antibody.Methods The ANA was detected by indirect immunofluorescence(IIF),the anti-SSA antibody and anti-SSB antibody were detected by Western-blot,and the hemoglobin(Hb),white blood cell(WBC),platelet(PLT),neutrophil percentage(NE%)and lymphocyte percentage(LY%)were detected by XE-2100 automatic instrument.Results Compared with the control group with negative ANA fluorescence,SSA and SSB,the hematological parameters Hb,WBC and PLT were significantly reduced in the patients with positive ANA,anti-SSA antibody and anti-SSB antibody,and there was significant difference between the two groups(P<0.05).Among those patients,53 patients(29.94%)were only Hb reducing,59 patients(33.33%)were only WBC reducing,24 patients(13.56%)were merely PLT reducing.Both Hb and WBC decreasing in 46 patients,about 10.50%,both WBC and PLT decreasing in 12 patients,about 6.78%,both Hb and PLT decreasing in 26 patients,about 14.59%.12 patients with Hb,WBC and PLT all cutting down,about 6.78%.Conclusion The changes of blood cell analysis parameters caused by abnormal injury in patients with positive autoantibodies in the blood system are of certain significance for the diagnosis and treatment of clinical autoimmune diseases.