Sysmex XN全自动血细胞分析仪在体液细胞计数及分类中的应用

目的评价Sysmex XN全自动血细胞分析仪体液模式在脑脊液和胸腹水标本细胞计数及分类计数中的应用价值。方法收集2019年7-8月该院住院患者体液标本341例,其中脑脊液标本138例及胸腹水标本203例,应用Sysmex XN全自动血细胞分析仪(仪器法)和改良牛鲍计数板(手工法)分别分类计数,对两种方法所测结果进行比较。结果仪器法与手工法对脑脊液标本有核细胞计数及红细胞计数一致性较好(ICC=0.985、0.994,均P<0.05),仪器法与手工法对脑脊液标本有核细胞分类计数中单个核细胞及多个核细胞计数一致性较好(ICC=0.917、0.946,均P<0.05);仪器法与手工法对胸腹水标本中有核细胞计数及红细胞计数一致性较好(ICC=0.960、0.996,均P<0.05);仪器法与手工法对胸腹水标本有核细胞分类计数中单个核细胞、多个核细胞及间皮细胞计数一致性较好(ICC=0.894、0.937、0.758,均P<0.05)。结论Sysmex XN全自动血细胞分析仪体液检测模式与手工法结果有较好的一致性,其操作简单、迅速,结果准确、可靠,重复性好,在体液细胞检测中具有较高的临床应用价值。

Application of Sysmex XN automated blood analyzer in the cell count and cell differential count of body-fluid sample

Objective To evaluate the application value of Sysmex XN automated blood analyzer in the cell count and cell differential count of cerebrospinal fluid and pleural effusion specimens.Methods A total of 341 samples,including 138 cases of cerebrospinal fluid specimens and 203 cases of pleural effusion specimens from hospitalized patients were collected,from July to August 2019 in this hospital.All specimens were differential counted by Sysmex XN automated blood cell analyzer(instrumental method)and modified neubauer hemocytometer(manual method)respectively,and the results of the two methods were compared.Results The consistency of nuclear cell count and red cell count of cerebrospinal fluid specimens was good between instrumental method and manual method(ICC=0.985,0.994,all P<0.05).There was a good consistency between instrumental method and manual method in the differential count of mononuclear cells and polynuclear cells in cerebrospinal fluid specimens(ICC=0.917,0.946,all P<0.05).The consistency of nuclear cell count and red blood cell count of pleural effusion specimens was good between instrumental method and manual method(ICC=0.960,0.996,all P<0.05).There was a good consistency between instrumental method and manual method in the differential count of mononuclear cells,polynuclear cells and mesothelial cells in of pleural effusion specimens(ICC=0.894,0.937,0.758,all P<0.05).Conclusion The body-fluid model of Sysmex XN automated blood analyzer has good consistency with the results of manual method in the cell count and cell differential count of cerebrospinal fluid and pleural effusion specimens.It is simple,rapid,accurate,reliable and repeatable,and has high clinical application value in the detection of body fluid cells.