miRNA-192-5p在多发性骨髓瘤中的表达水平及其调控机制研究

目的 探讨miR-192-5 p在多发性骨髓瘤中的表达水平及其调控机制.方法 选取骨髓瘤细胞株U266作为研究对象,利用荧光定量PCR检测多发性骨髓瘤细胞中miR-192-5 p的表达水平;使用生物信息学网站预测miR-192-5 p潜在靶基因USP1并利用双荧光素酶报告实验验证其靶向关系;利用细胞转染实验对多发性骨髓...

Expression of miRNA-192-5p in multiple myeloma and its regulatory mechanism

Objective To explore the expression of miR-192-5p in multiple myeloma and its regulatory mechanism.Methods The Myeloma cell line U266 was selected as the object of study,and the expression of miR-192-5p in multiple myeloma cells was detected by fluorescence quantitative PCR.The potential target gene USP1 of miR-192-5p was predicted by bioinformatics website and its targeting relationship was verified by double luciferase reporting experiment.Multiple myeloma cell lines were transfected with miR-192-5p mimics,miR-192-5p inhibitor and associated control groups by cell transfection experiment.The proliferation and apoptosis of the transfected cells were detected by CCK-8 and flow cytometry respectively.The expression of USP1 protein in the transfected cells was detected by Western blot.Results The expression of miR-192-5p in the transfected multiple myeloma cells was lower than that in the normal bone marrow cells(P<0.01).The over-expression of miR-192-5p inhibited the proliferation,migration and invasion of myeloma cells and promoted the apoptosis of myeloma cells.The results of target gene prediction and validation experiments showed that USP1 was the target gene of,and the miR-192-5p could significantly reduce the expression of USP1 protein in myeloma cells.Conclusion miR-192-5p may play a role as a tumor suppressor gene in multiple myeloma and inhibit the development of multiple myeloma by targeting USP1.