子宫内膜癌患者血清及癌组织中microRNA-195的表达水平及意义

目的观察microRNA-195在子宫内膜癌患者血清及组织中的表达情况,并分析其诊断价值及与病理学指标的关系。方法子宫内膜癌癌组织及对应肿瘤边缘2~5 cm处的癌旁组织来源于2018年1—4月石家庄心脑血管病医院接受子宫内膜癌分期手术治疗的60例患者(病例组),并抽取其术前外周静脉血;同时抽取同期入院行妇科体检的30例健康志愿者作为对照(健康组)。采用实时荧光定量PCR检测血清及组织中microRNA-195的相对表达量,并分析其与病理学指标的关系。结果与健康组相比,病例组血清microRNA-195表达水平明显低,差异有统计学意义(P<0.05)。血清microRNA-195对诊断子宫内膜癌的受试者工作特征曲线下面积为0.778(95%CI:0.692~0.864),确定最佳阈值后诊断子宫内膜癌的灵敏度、特异度分别为76.67%、73.33%。与癌旁组织相比,子宫内膜癌癌组织中microRNA-195相对表达量明显低,差异有统计学意义(P<0.05)。不同的年龄、病理类型、病理分级、ER、PR表达强度的子宫内膜癌癌组织microRNA-195表达水平比较,差异无统计学意义(P>0.05);而microRNA-195表达水平Ⅰ期癌组织>Ⅱ期癌组织>Ⅲ期癌组织,浸润深度≥1/2肌层的癌组织microRNA-195表达水平明显高于浸润深度<1/2肌层的癌组织,Her-2阴性的癌组织癌组织microRNA-195表达水平明显高于Her-2阳性的癌组织,差异均有统计学意义(P<0.05)。结论microRNA-195在子宫内膜癌患者血清及癌组织中呈低表达,对子宫内膜癌的诊断有一定价值,且可能对子宫内膜癌的发生发展有一定促进作用。

Expression level and significance of microRNA-195 in serum and cancerous tissue of endometrial cancer patients

Objective To observe the expression of microRNA-195 in serum and cancerous tissue of endometrial cancer patients,to analyze the diagnostic value and its relationship with pathological indexes.Methods Cancerous tissues of endometrial cancer and para-carcinoma tissues at 2 to 5 cm from the edge of the tumor were obtained from 60 patients(case group)who underwent stage operation for endometrial cancer in Shijiazhuang Cardiovascular and Vascular Disease Hospital from January 2018 to April 2018.The preoperative peripheral venous blood was extracted.At the same time,30 healthy volunteers undergoing gynecological examination in the hospital during the same period were enrolled as controls(healthy group).Real-time fluorescent quantitative PCR was performed to detect the relative expression of microRNA-195 in serum and tissues,and its relationship with pathological indexes was analyzed.Results Compared with healthy group,expression level of serum microRNA-195 in case group was significantly lower,the difference was statistically significant(P<0.05).The area under the receiver operating characteristic curve of serum microRNA-195 for the diagnosis of endometrial cancer was 0.778(95%CI:0.692 to 0.864).After determining optimal threshold,sensitivity and specificity for diagnosis of endometrial cancer were 76.67%and 73.33%respectively.Compared with para-carcinoma tissue,the relative expression of microRNA-195 in cancerous tissue of endometrial cancer was significantly lower,the difference was statistically significant(P<0.05).There were no significant differences in the expression levels of microRNA-195 in cancerous tissue of endometrial cancer with different ages,different pathological types,different pathological grades and different expression intensity of ER and PR(P>0.05).Arranging expression level of microRNA-195 from high to low,the corresponding order was stageⅠcancerous tissues,stageⅡand stageⅢ.The expression level of microRNA-195 in cancerous tissue with infiltration depth of or larger than 1/2 of muscle layer was significantly higher than that with infiltration depth less than 1/2 of muscle layer,The expression level of microRNA-195 in cancerous tissue with negative Her-2 was significantly higher than that with positive Her-2,the difference was statistically significant(P<0.05).Conclusion microRNA-195 is lowly expressed in serum and cancerous tissue of endometrial cancer patients.It is of certain diagnostic value for endometrial cancer,which may promote the occurrence and development of endometrial cancer.