miRNA-17-5p、miRNA-103-3p及miRNA-144-3p在急性缺血性脑卒中中的表达及诊断价值研究

目的探讨微小RNA(miRNA)-17-5p、miRNA-103-3p及miRNA-144-3p在急性缺血性脑卒中(AIS)中的表达及临床诊断价值。方法分别收集该院50例非脑卒中患者和50例AIS患者的血清标本作为对照组和病例组。采用实时荧光定量PCR法检测血清中miRNA-17-5p、miRNA-103-3p及miRNA-144-3p的表达水平,同时将血清中miRNA-17-5p、miRNA-103-3p及miRNA-144-3p的水平与研究对象的临床诊断价值进行对比分析。结果病例组血清miRNA-17-5p、miRNA-103-3p及miRNA-144-3p表达水平均显著高于对照组(P<0.001)。AIS患者血清miRNA-17-5p、miRNA-103-3p及miRNA-144-3p与美国国立卫生院卒中量表(NIHSS)评分呈显著正相关(r=0.605、0.667、0.710,均P<0.05)。miRNA-17-5p、miRNA-103-3p、miRNA-144-3p及三者联合区分AIS患者与非AIS的受试者工作特征曲线下面积分别为0.846(95%CI:0.757~0.935,P<0.001)、0.866(95%CI:0.788~0.945,P<0.001)、0.935(95%CI:0.888~0.982,P<0.001)和0.976(95%CI:0.953~0.999,P=0.000);灵敏度和特异度分别为84%和92%、84%和88%、92%和86%、92%和94%。结论miRNA-17-5p、miRNA-103-3p及miRNA-144-3p是AIS诊断的潜在标志物。

Expressions and clinical diagnostic values of miRNA-17-5p,miRNA-103-3p and miRNA-144-3p in acute ischemic stroke

Objective To investigate the expressions and clinical values of miRNA-17-5p,miRNA-103-3p and miRNA-144-3p in acute ischemic stroke(AIS).Methods Serum samples from 50 non AIS patients and 50 AIS patients in the hospital were collected as control group and case group.Real-time quantitative PCR was used to detect the expressions of miRNA-17-5p,miRNA-103-3p and miRNA-144-3p in serum,and the clinical diagnosis values of miRNA-17-5p,miRNA-103-3p and miRNA-144-3p were statically analyzed.Results The serum levels of miRNA-17-5p,miRNA-103-3p and miRNA-144-3p in the case group were significantly higher than those in the control group(P<0.001).Serum miRNA-17-5p,miRNA-103-3p and miRNA-144-3p were positively correlated with NIHSS(r=0.605,0.667,0.710,all P<0.05).The area under the working characteristic curve of the subjects who distinguish AIS patients from non AIS patients by miRNA-17-5p,miRNA-103-3p,miRNA-144-3p and combined detection of three indicators was 0.846(95%CI:0.757—0.935,P<0.001),0.866(95%CI:0.788—0.945,P<0.001),0.935(95%CI:0.888—0.982,P<0.001),0.976(95%CI:0.953—0.999,P=0.000),the sensitivity and specificity were 84%and 92%,84%and 88%,92%and 86%,92%and 94%,respectively.Conclusion miRNA-17-5p,miRNA-103 and miRNA-144-3p are potential markers for AIS diagnosis.