CD4+CD25+CD127Low调节性T细胞在原发性肝癌患者外周血的表达及临床意义

目的观察原发性肝癌患者经治疗前、后外周血CD4+CD25+CD127(CD127Low)调节性T细胞(Treg)的数量变化。方法采用流式细胞术检测30例原发性肝癌患者外周血CD4^+CD25^+CD127^LowTreg在CD4+T细胞的比例,以180例体检健康者作为健康对照组,分析肝癌患者治疗前、后CD4^+CD25^+CD127^Low Treg细胞表达的变化。结果原发性肝癌治疗前CD4^+CD25^+CD127^LowTreg细胞百分比为(6.21±1.53)%(<50岁)和(6.68±1.53)%(≥50岁),均显著高于健康对照组(同年龄段),差异有统计学意义(P=0.001、0.000),原发性肝癌患者治疗1个月有效者CD4^+CD25^+CD127^LowTreg细胞比例显著下降,差异有统计学意义(P=0.001)。结论CD4^+CD25^+CD127^LowTreg水平异常增高可能与原发性肝癌免疫功能调节紊乱有关,且其变化水平与临床病情的进展存在一定的相关性。

The expression and clinical significance of CD4+CD25+CD127Low regulatory T cells in peripheral blood of patients with primary carcinoma of liver

Objective To survey the quantity change of CD4^+CD25^+CD127^Low regulatory T lymphocyte cells(Treg)in the peripheral blood of patients with primary carcinoma of liver before and after treatment.Methods The percentage of CD4^+CD25^+CD127^Low Treg cells in CD4+T cells from 30 patients with primary liver cancer before and after treatment were detected by flow cytometry analysis,and were also compared with those of 180 healthy subjects as healthy control group.Results The percentage of CD4^+CD25^+CD127^LowTreg cells in the pretreatment of primary carcinoma of liver was(6.21±1.53)%(<50 years old)and(6.68±1.53)% (≥50 years old),both were significantly higher than those in the control group(P=0.001,0.000).CD4^+CD25^+CD127^Low Treg cells decreased significantly after effective treatments for a month(P=0.001).Conclusion The abnormal increase of CD4^+CD25^+CD127^LowTreg level may be related to the disorder of immune function in primary carcinoma of liver,and the changes in CD4^+CD25^+CD127^LowTregs may be tightly associated with the progress of clinical disease.