IL-17基因多态性与支气管哮喘患儿血清TIgE、ECP的关系分析

目的分析白细胞介素(IL)-17基因多态性与支气管哮喘患儿血清总IgE(TIgE)、嗜酸性粒细胞阳离子蛋白(ECP)的关系。方法将该院2018年1—12月收治的60例哮喘患儿设为哮喘组,同期于该院行健康体检的60例健康儿童设为对照组,采用PCR-限制性片段长度多态性分析法检测2组IL-17基因多态性,采用免疫比浊法、酶联免疫吸附试验法分别检测其血清TIgE、ECP水平,比较2组IL-17基因多态性、TIgE和ECP水平及不同基因型、不同等位基因型患儿血清TIgE、ECP水平。结果哮喘组中IL-17A-152G/A位点中AA型基因频数频率、A等位基因频数频率,IL-17F 7488T/C位点中TT型基因频数频率、T等位基因频数频率,血清TIgE、ECP水平均显著高于对照组(P<0.05)。哮喘组IL-17A-152G/A位点AA型基因血清TIgE显著高于AG、GG型(P<0.05)。IL-17A-152G/A位点A等位基因携带患儿血清TIgE显著高于非A等位基因携带患儿(P<0.05)。哮喘组IL-17F 7488T/C位点不同基因型、等位基因携带者间血清TIgE、ECP与对照组比较,差异无统计学意义(P>0.05)。结论IL-17A-152G/A基因多态性与哮喘易感性有关,变异等位基因A携带患儿哮喘风险更高,尤其是突变纯合子AA基因型,野生型TT纯合子与哮喘也有一定关联性;且IL-17基因多态性虽与血清ECP均无明显关联,但血清ECP在哮喘患儿中仍明显高表达。

Relationship between IL-17 gene polymorphism and serum TIgE and ECP in children patients with bronchial asthma

Objective To analyze the relationship between interleukin-17(IL-17)gene polymorphism and serum total IgE(TIgE)and eosinophil cationic protein(ECP)in children patients with bronchial asthma.Methods A total of 60 children patients with asthma admitted to the hospital from January 2018 to December 2018 were set as asthma group,and 60 healthy children admitted to the hospital for physical examination at the same period were set as control group.The IL-17 gene polymorphism in the two groups was detected by polymerase chain reaction-restriction fragment length polymorphism.Serum TIgE and ECP were detected by immunoturbidimetry and enzyme-linked immunosorbent assay.The IL-17 gene polymorphism and levels of serum TIgE and ECP in the two groups and levels of serum TIgE and ECP of children patients with different genotypes and different allelotypes were compared.Results The AA genotype frequency and A allele frequency in IL-17A-152G/A locus,TT gene frequency and T allele frequency in IL-17F 7488T/C locus and levels of serum TIgE and ECP in asthma group were significantly higher than those in control group(P<0.05).The serum TIgE of AA gene in IL-17A-152G/A locus in asthma group was significantly higher than that of AG and GG(P<0.05).Serum TIgE of IL-17A-152G/A locus A allele carriers was significantly higher than that of non-A allele carriers(P<0.05).There were no statistically significant differences in the serum TIgE and ECP between asthma group and control group with different genotypes and alleles of IL-17F 7488T/C locus(P>0.05).Conclusion IL-17A-152G/A gene polymorphism is associated with asthma susceptibility.Children patients with high variant A allele has high risk of asthma,especially the homozygous AA genotype.Wild type TT homozygote is also associated with asthma.There was no significant association between IL-17 gene polymorphism and serum ECP,but serum ECP is still highly expressed in children patients with asthma.