FHL2与PBX3在宫颈癌组织中表达与临床病理特征及预后的相关性

目的 探讨四个半LIM结构域蛋白2(FHL2)FHL2与前B细胞白血病同源盒基因3(PBX3)在宫颈癌组织中的表达与临床特征及预后的相关性。方法 选取该院2013年12月至2015年12月收治的55例宫颈癌患者作为研究对象,回顾性分析患者的临床资料,将收集到的患者标本分组,55例癌组织标本作分为研究组。将55例癌旁组织标本(距肿瘤边缘4cm处)作为对照组。并采用免疫组化法检测所有患者的FHL2与PBX3蛋白的表达,分析FHL2与PBX3蛋白表达与宫颈癌患者临床病理特征的关系,并采用Cox比例风险分析影响宫颈癌患者预后的相关因素。结果 研究组FHL2、PBX3蛋白阳性表达明显高于对照组,差异有统计学意义(P<0.001);FHL2、PBX3蛋白表达与宫颈癌患者的年龄、病理类型等临床特征不相关(P>0.05),但与宫颈癌患者的肿瘤分化程度、淋巴结转移、浸润深度以及病理分级相关(P<0.05),当肿瘤分化程度越低、有淋巴转移、浸润深度越深以及病理程度越高时,FHL2、PBX3蛋白阳性表达率越高(P<0.05);宫颈癌患者平均中位生存期20个月,其中FHL2阳性表达和阴性表达患者的中位生存期分别为18、30个月,PBX3阳性表达和阴性表达患者的中位生存期分别为17、32个月中位生存期;经单因素分析得出:肿瘤低分化、淋巴结转移、浸润深度>1/2、病理分级G3、FHL2、PBX3阳性表达的患者生存时间均显著缩短,差异有统计学意义(P<0.05);经多因素Cox比例风险回归模型分析得出:肿瘤低分化、淋巴结转移、浸润深度>1/2、病理分级G3、FHL2、PBX3阳性表达均为影响宫颈癌患者预后的独立影响因素(P<0.05)。结论 FHL2与PBX3蛋白在宫颈癌组织中的表达水平升高,且表达水平与宫颈癌患者的临床特征显著相关,对于宫颈癌的发生、发展以及预后均有着重要的临床意义。

Correlation between FHL2 and PBX3 expression and clinicopathological features and prognosis of patients with cervical cancer

Objective To investigate the correlation between four and a half LIM domains protein 2(FHL-2)and pre-B-cell leukemia homeobox 3(PBX3)expression and clinicopathological features and prognosis of patients with cervical cancer.Methods The clinical data of 55 patients with cervical cancer admitted to the hospital from December 2013 to December 2015 were retrospectively analyzed.The tissues collected from the patients were divided into study group(cancer tissues)and control group(para-carcinoma tissues,4cm from the edge of the tumor)according to the data,each with 55 specimens.The expressions of FHL2 and PBX3 protein were detected by immunohistochemistry,and its correlation with clinicopathological features and prognosis was analyzed.Then the Cox proportional hazard analysis was used to determine the prognostic factors of cervical cancer patients.Results The positive expression of FHL2 and PBX3 protein in the study group was significantly higher than that in the control group(P<0.001).The expressions of FHL2 and PBX3 protein had no correlation with age and pathological type of cervical cancer patients(P>0.05),but was positively correlated with the degree of differentiation,lymph node metastasis,infiltration depth and pathological grade of cervical cancer patients(P<0.05).The median survival time of patients with cervical cancer was 20 months.The median survival time of patients with FHL2 positive expression and negative expression was 18 and 30 months respectively.The median survival time of patients with PBX3 positive expression and negative expression was 17 and 32 months respectively.Univariate analysis showed that the survival time of patients with low differentiation,lymph node metastasis,depth of invasion>1/2,pathological grade G3,FHL2 and PBX3 positive expression was significantly shortened(P<0.05).Multivariate Cox proportional risk regression analysis showed that poor differentiation,lymph node metastasis,depth of invasion>1/2,pathological grade G3,FHL2,PBX3 positive expression were independent factors affecting the prognosis of cervical cancer patients(P<0.05).Conclusion The expression levels of FHL2 and PBX3 protein in cervical cancer tissues are increased,which is significantly related to the clinical characteristics of cervical cancer patients,indicating that the two indicators have important clinical significance for the occurrence,development and prognosis of cervical.