七肽TVNFKLY和LPQRLRT在儿童肺炎支原体肺炎中的诊断价值

目的探讨七肽TVNFKLY和LPQRLRT在儿童肺炎支原体肺炎中的诊断价值。方法选取2016年5月至2019年5月该院收治的儿童肺炎支原体肺炎患儿120例为支原体组、非儿童肺炎支原体肺炎患儿120例为非支原体组和体检健康儿童120例为对照组。酶联免疫吸附测定试验检测各组血清中七肽TVNFKLY和LPQRLRT水平。采用受试者工作特征曲线(ROC曲线)的曲线下面积(AUC)分析七肽TVNFKLY和LPQRLRT检测在儿童肺炎支原体肺炎中的诊断意义。结果儿童肺炎支原体肺炎患儿血清中七肽TVNFKLY和LPQRLRT水平高于非儿童肺炎支原体肺炎患儿和体检健康儿童,差异有统计学意义(P<0.05)。血清中七肽TVNFKLY和LPQRLRT单向诊断儿童肺炎支原体肺炎的灵敏度分别为95.00%和92.50%,特异度分别为87.08%和90.42%,准确度分别为89.72%和91.11%。血清中七肽TVNFKLY和LPQRLRT联合对儿童肺炎支原体肺炎的诊断的灵敏度为92.50%,特异度为90.83%,准确度为91.39%。结论血清中七肽TVNFKLY和LPQRLRT水平检测可作为诊断儿童肺炎支原体肺炎的潜在标志。

Diagnostic value of heptapeptide TVNFKLY and LPQRLRT in children with Mycoplasma pneumoniae pneumonia

Objective To investigate the diagnostic value of heptapeptide TVNFKLY and LPQRLRT in children with Mycoplasma pneumoniae pneumonia.Methods A total of 120 children with Mycoplasma pneumoniae pneumonia admitted to a hospital from May 2016 to May 2019 were enrolled in the Mycoplasma group,120 children with non-Mycoplasma pneumoniae pneumonia were non-Mycoplasma group and 120 healthy children were used as control group.The levels of heptapeptide TVNFKLY and LPQRLRT in the serum of three groups of subjects were measured by enzyme-linked immunosorbent assay.The diagnostic significance of heptapeptide TVNFKLY and LPQRLRT in children with Mycoplasma pneumoniae pneumonia was analyzed by AUC of receiver operating characteristic curve(ROC curve).Results The levels of heptapeptide TVNFKLY and LPQRLRT in children with Mycoplasma pneumoniae pneumonia were higher than those in children with non-Mycoplasma pneumoniae pneumonia and healthy children,the difference was statistically significant(P<0.05).The sensitivity of the seven-peptide TVNFKLY and LPQRLRT in the diagnosis of children with Mycoplasma pneumoniae pneumonia was 95.00%and 92.50%,the specificity was 87.08%and 90.42%,respectively,and the accuracy was 89.72%and 91.11%,respectively.The sensitivity of the combination of heptapeptide TVNFKLY and LPQRLRT in the diagnosis of children with Mycoplasma pneumoniae pneumonia was 92.50%,the specificity was 90.83%,and the accuracy was 91.39%.Conclusion The detection of the levels of heptapeptide TVNFKLY and LPQRLRT in serum can be used as a potential marker for the diagnosis of children with Mycoplasma pneumoniae pneumonia.