肠道益生菌补充对酒精高糖高脂致肝损伤大鼠肝功能和肠道菌群的影响

目的 探究肠道益生菌补充对酒精、高糖、高脂致肝损伤大鼠肝功能和肠道菌群的影响.方法 高糖高脂饮食联合白酒灌胃处理制备酒精、高糖、高脂致肝损伤大鼠模型,并分为空白对照组、模型组、低剂量肠道益生菌组(20×108 CFU/kg)和高剂量肠道益生菌组(40×108 CFU/kg).灌服白酒7周后,算大鼠脏体比、血清乙醇体积分...

Effects of intestinal probiotic supplementation on liver function and intestinal flora in rats with liver injury induced by alcoholic high glucose and high fat

Objective To explore the effects of intestinal probiotic supplementation on liver function and intestinal flora in rats with liver injury induced by alcoholic,high glucose and high fat.Methods The rat model of liver injury induced by high glucose and high fat combined with liquor gavage was prepared,and divided into blank control group,modelgroup,low-dose intestinal probiotic group(20×10⁸ CFU/kg)and high-doseintestinalprobiotic group(40×10⁸ CFU/kg).After 7 weeks of liquor gavage,the organ-body ratio and serum ethanol volume fraction were measured.The alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(AKP),triglyceride(TG),total cholesterol(TC),low-density lipoprotein sterol(LDL-C)and high-density lipoprotein cholesterol(HDL-C)were detected.The tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),transforming growth factor-β1(TGF-β1),diamine oxidase(DAO)and D-lactic acid(D-LA)of liver tissues were detected by enzyme linked immunosorbent assay(ELISA).The Lactobacillus,Bifidobacterium,Escherichia coli and Enterococcus in rat feces were cultured by the method of feces plate cultivation,and the quantity of various bacteria in rat feces was calculated.The expression of NF-κB protein in liver tissue was measured by Western Bolt.Results Compared to the blank control group,the organ-body ratio of model group was significantly increased,the blind body ratio was significantly decreased;the ethanol content in body,ALT,AST,AKP,TG,TC,LDL-C,TNF-α,IL-6,TGF-β1,DAO and D-LA of model group were increased significantly while the HDL-C was decreased significantly,and the NF-κB protein expression of model group was increased significantly(P<0.01),and there were no significant changes in all the indexes in low-dose group and high-dose group(P>0.05).Compared to the model group,the organ-body ratio of low-dose group and high-dose group were significantly decreased,the blind body ratio was significantly increased,and the ALT,AST,AKP,TG,TC,LDL-C,TNF-α,IL-6,TGF-β1,DAO and D-LA were significantly decreased while the HDL-C of low-dose group and high-dose group were significantly increased,and the NF-κB protein expression of low-dose group and high-dose group was significantly decreased(P<0.01).Conclusion Intestinal probiotic supplementation can protect the liver function and intestinal flora by regulating the types and quantity of intestinal probiotics,reducing the expression of NF-κB protein and inflammatory factors in serum in rats with liver injury induced by alcoholic high glucose and high fat.