BioPlex 2200全自动免疫分析仪检测血清中抗双链DNA抗体的临床应用

目的分析比较BioPlex 2200全自动免疫分析仪和酶联免疫吸附测定(ELISA)检测血清中抗双链DNA(dsDNA)抗体的灵敏度、特异度及其优缺点。方法采用BioPlex 2200和ELISA检测101例纳入研究者的血清抗dsDNA抗体。纳入研究者中49例为临床确诊的系统性红斑狼疮患者,52例作为对照(包括32例其他结缔组织病患者和20例体检健康者)。结果BioPlex 2200检测抗dsDNA抗体的灵敏度为57.14%,特异度为94.23%;ELISA检测抗dsDNA抗体的灵敏度为53.06%,特异度为96.15%。两种方法总体符合率为95.05%,χ2检验分析,差异无统计学意义(χ2=78.69,P>0.05),Kappa值评价一致性较好(Kappa=0.880)。BioPlex 2200检测抗dsDNA抗体,3组抗dsDNA抗体水平比较,差异有统计学意义(F=61.52,P<0.05)。结论BioPlex 2200和ELISA检测血清抗dsDNA抗体结果有较好的一致性,检测迅速,值得临床推广应用。

Clinical application of BioPlex 2200 automatic immunoanalyzer to detect anti-double-stranded DNA antibodies in serum

Objective To analyze and compare the sensitivity,specificity,advantages and disadvantages of the detection of anti-double-stranded DNA(dsDNA)antibodies in serum by BioPlex 2200 automatic immunoassay analyzer and enzyme-linked immunosorbent assay(ELISA).Methods The serum anti-dsDNA antibodies of 101 subjects were detected by Bioplex 2200 and ELISA,including 49 patients with clinically confirmed systemic lupus erythematosus and 52 cases as a control(including 32 other connective tissue diseases patients and 20 healthy people).Results The sensitivity of BioPlex 2200 to detect anti-dsDNA antibodies was 57.14%and the specificity was 94.23%;the sensitivity of ELISA to detect anti-dsDNA antibodies was 53.06%and the specificity was 96.15%.The overall coincidence rate of the two methods was 95.05%,and theχ2 test analysis showed no significant difference(χ2=78.69,P>0.05).The Kappa value was consistent(Kappa=0.880).The levels of anti dsDNA antibody was detected by Bioplex 2200,the difference was statistically significant(F=61.52,P<0.05).Conclusion BioPlex 2200 and ELISA have good consistency in the detection of serum anti-dsDNA antibodies,and the detection is rapid,which is worthy of clinical application.