不同类型肠易激综合征患者血清miRNA-144、miRNA-29a和miRNA-200a水平的临床意义

目的探讨不同类型肠易激综合征(IBS)患者血清微小RNA(miRNA)-144、miRNA-29a和miRNA-200a表达水平的相关性及临床意义,为IBS的病因学研究提供理论依据。方法采用病例抽样的方法选取2015年10月至2018年6月该院IBS患者110例,选取同期110例体检健康者为健康对照组。受试者采样前12 h低蛋白饮食,每位受试者取10 mL静脉血样本,静脉血3000 r/min离心后取上清。采用实时荧光定量反转录PCR测定受试者miRNA-144、miRNA-29a和miRNA-200a表达水平。结果混合型IBS的miRNA-144、miRNA-29a和miRNA-200a表达水平均显著高于其他3种亚型(P<0.05),不确定型IBS的miRNA-144、miRNA-29a和miRNA-200a表达水平均显著高于便秘型IBS及腹泻型IBS(P<0.05)。混合型、不确定型IBS患者的miRNA-144、miRNA-29a、miRNA-200a的表达水平两两之间均存在相关性且有统计学意义(P<0.05),但对于单纯腹泻或者便秘型IBS患者来说,仅miRNA-29a与miRNA-200a的表达水平存在统计学相关性(P<0.05)。结论混合型、不确定型IBS患者血清miRNA-144、miRNA-29a和miRNA-200a表达水平较其他2种亚型高,且血清miRNA-144、miRNA-29a和miRNA-200a表达两两之间具有显著相关性。

Clinical significance of serum miRNA-144,miRNA-29a,miRNA-200a levels in patients with different types of irritable bowel syndrome

Objective To explore the correlation and clinical significance of serum microRNA(miRNA)-144,miRNA-29a,miRNA-200a expression in different types of irritable bowel syndrome(IBS)patients,and provide a theoretical basis for the etiology of IBS.Methods Totally 110 IBS patients From October 2015 to Junc 2018 were selected by case sampling method,and 110 healthy people were selected as control group.The subjects were fed a low protein diet 12 hours before sampling,10 mL of venous blood samples were taken from each subject,and the supernatant was taken after centrifugation of the venous blood at 3000 r/min.The expression levels of miRNA-144,miRNA-29a and miRNA-200a were measured by real-time fluorescence quantitative PCR.Results The expression levels of miRNA-144,miRNA-29a and miRNA-200a in mixed IBS were significantly higher than those in the other three subtypes(P<0.05).The expression of miRNA-144,miRNA-29a and miRNA-200a in uncertain IBS was significantly higher than that in constipation IBS and diarrhea IBS(P<0.05).The expression levels of miRNA-144,miRNA-29a and miRNA-200a in patients with mixed and uncertain IBS were statistically correlated(P<0.05),but only miRNA-29a and miRNA-200a were statistically correlated in patients with simple diarrhea or constipation IBS(P<0.05).Conclusion The expression levels of miRNA-144,miRNA-29a and miRNA-200a in mixed and uncertain IBS patients were higher than those in other two subtypes,and there was significant correcation between miRNA-144,miRNA-29a and miRNA-200a in serum.