miRNA-10b与乳腺癌相关肿瘤标志物在早期乳腺癌中诊断效能比较

目的探讨微小核糖核酸-10b(miRNA-10b)与肿瘤标志物癌胚抗原(CEA)、糖类抗原153(CA153)、糖类抗原199(CA199)和糖类抗原125(CA125)在早期乳腺癌诊断中的价值。方法选取2015年7月至2017年12月该院诊治的乳腺疾病女性患者92例,其中乳腺恶性肿瘤患者50例(早期乳腺癌30例、晚期乳腺癌20例)、乳腺良性疾病患者42例,另选健康对照者50例。采用电化学发光法分别检测乳腺癌患者、乳腺良性疾病患者和健康对照者血清中CEA、CA153、CA199和CA125的水平。采用荧光定量PCR法检测外周血中miRNA-10b水平。结果CEA、CA199及CA125在乳腺癌良性疾病组患者血清中表达水平略升高,但与健康对照组比较,差异无统计学意义(P>0.05)。早期乳腺癌组患者血清中仅CA153、miRNA-10b表达水平明显升高(P<0.05)。晚期乳腺癌组患者血清CEA、CA153、CA199、CA125和miRNA-10b水平均升高(P<0.05)。miRNA-10b和CA153的受试者工作特征曲线(ROC曲线)下面积(AUC)、灵敏度和特异度均优于CEA、CA199和CA125。结论CA153和miRNA-10b可作为早期乳腺癌筛查的血清学指标,诊断准确率高。CEA、CA125和CA199不可用于早期乳腺癌肿瘤筛查。

Comparison of miR NA-10b and breast cancer related tumor markers in the diagnosis of early breast cancer

Objective To investigate the value of microRNA-10b(miRNA-10b),carcinoembryonic antigen(CEA),carbohydrate antigen 153(CA153),carbohydrate antigen 199(CA199)and carbohydrate antigen 125(CA125)in the diagnosis of early breast cancer.Methods From July 2015 to December 2017,92 female patients with breast diseases were selected,including 50 patients with breast cancer(early breast cancer 30 cases,late breast cancer 20 cases),42 patients with breast benign diseases,and 50 cases of healthy controls.The levels of CEA,CA153,CA199 and CA125 in sera of breast cancer patients,breast benign disease patients and healthy controls were detected by electrochemiluminescence.The level of miRNA-10b in peripheral blood was detected by fluorescence quantitative PCR.Results The levels of CEA,CA199 and CA125 in the sera of breast cancer patients with benign disease groups increased slightly,but there was no significant difference between the two groups(P>0.05).The expression of CA153 and miRNA-10b in serum of patients with early breast cancer group was significantly increased(P<0.05).The levels of CEA,CA153,CA199,CA125 and miRNA-10b were all increased in patients with advanced breast cancer group(P<0.05).The area under curve(AUC),sensitivity and specificity of miRNA-10b and CA153 were superior to CEA,CA199 and CA125.Conclusion CA153 and miRNA-10b can be used as serological markers for early breast cancer screening,and the diagnostic accuracy is high.CEA,CA125 and CA199 are not suitable for screening early breast cancer.