7种数据库工具对MRPL13蛋白在乳腺癌中的表达、功能分析及预后价值研究

目的通过Oncomine、cBioPortal、癌症细胞系百科全书(CCLE)和The human protein atlas等多个数据库,探讨线粒体核糖体蛋白L13(MRPL13)在乳腺癌组织与正常乳腺组织的表达差异,并进一步分析MRPL13表达与乳腺癌患者预后的关系。方法首先利用Oncomine和cBioPortal数据库分析MRPL13在乳腺癌和正常乳腺组织中的表达差异,用CCLE数据库分析MRPL13在乳腺癌细胞系中的表达情况,并利用The human protein atlas数据库观察MRPL13蛋白水平的表达变化。接着,通过String数据库建立MRPL13的共表达网络,并且利用DAVID数据库对共表达网络进行GO生物学功能和KEGG信号通路富集分析。最后利用cBioPortal和Kaplan-Meier Plot在线分析网络工具探索分析MRPL13表达变化与乳腺癌患者生存期的关系。结果Oncomine数据库发现MRPL13在乳腺癌中高表达(P=5.55×10-9),cBioPortal数据库显示816例乳腺癌患者样本中有43%的MRPL13基因表达上调。同样在CCLE数据库也发现MRPL13在乳腺癌细胞系中高表达,3个数据库结果一致。The human protein atlas数据库发现MRPL13蛋白中等程度表达且主要集中表达在细胞质与细胞膜内。cBioPortal和Kaplan-Meier Plotter网络在线分析均显示乳腺癌患者中MRPL13高表达能显著降低乳腺癌患者生存期,差异均有统计学意义(P<0.05)。结论相较于正常组织,MRPL13在乳腺癌中高表达,且提示乳腺癌患者预后不良,可作为乳腺癌患者预后评估的分子标志物之一。

Biological analysis and prognostic value of MRPL13 in breast cancer:a study based on several databases and bioinformatics

Objective To explore the expression of mitochondrial ribosomal protein L13(MRPL13)between breast carcinoma and normal breast tissues based on Oncomine,cBioPortal,cancer cell line encyclopedia(CCLE)and the human protein atlas database,and analyze the relationship between the expression of MRPL13 and prognosis of breast carcinoma patients.Methods Firstly,through both Oncomine and cBioPortal database to find out the different expression of MRPL13 in breast carcinoma and normal tissue and analyze the expression of MRPL13 in cell line by CCLE database.After that,through the human protein atlas database to analyze the protein expression level of MRPL13 in breast cancer.Then to establish MRPL13 co-expression network by String database and to conduct GO enrichment and KEGG pathway analysis for this network by DAVID database.Next,through cBioPortal and Kaplan-Meier Plot to analyze the overall survival of breast cancer patients with MRPL13.Results MRPL13 was obviously over-expressed in breast cancer based on Oncomine database(P=5.55×10-9).cBioPortal database showed that among about 43%of the 816 samples MRPL13 was up-regulated in breast cancer.CCLE database also found MRPL13 was over-expression in cell line of breast cancer.Through the human protein atlas database to identify MRPL13 protein was moderate expression in breast cancer and mainly expression in cytoplasmic or membranous.Finally,both cBioPortal and Kaplan-Meier Plotter reveal patients with the over-expressed level of MRPL13 might obviously reduce survival rate(P<0.05).Conclusion over-expressed MRPL13 in breast cancer may predict lower survival,which also could regard as one of the prognostic biomarkers for breast cancer patients.