血清、脑脊液中S-100B、NSE水平与缺血性脑卒中的研究

目的探讨血清、脑脊液中S-100B、神经元特异性烯醇化酶(NSE)与缺血性脑卒中的关系。方法将无特定病原体(SPF)级48只SD大鼠随机编号分为8组,每组6只,手术后8 h做行为学评价。按照不同时间段,术后8 h、12 h、1 d、3 d、5 d、7 d、21 d腹腔取血及抽取脑脊液,检测S-100B蛋白及NSE水平;进行2,3,5-氯化三苯基四氮唑(TTC)大脑染色及行为学功能评价并分析其相关性。结果SD大鼠血清及脑脊液中S-100B及NSE在不同时间段呈趋势性变化;脑脊液中S-100B、NSE水平增高时间均早于血清中S-100B、NSE水平增高时间;脑损伤后12 h,脑脊液中NSE水平即可达到峰值,血清中S-100B、NSE水平增加,3 d后达到峰值,7 d后S-100B、NSE水平与对照组比较,差异无统计学意义(P>0.05);血清和脑脊液中S-100B、NSE水平与梗死面积、神经功能评分均呈正相关(P<0.05)。结论脑脊液及血清中S-100B及NSE均能反应脑损伤程度,作为缺血性脑卒中的生物学指标,可进一步为临床患者提供血液及脑脊液标本采集的时间窗口。

Study on the levels of S-100B and NSE in serum and cerebrospinal fluid and ischemic stroke

Objective To investigate the relationship between S-100B,neuron-specific enolase(NSE)in serum and cerebrospinal fluid and ischemic stroke.Methods A total of 48 SD rats without specific pathogen(SPF)were randomly divided into 8 groups,6 rats in each group,and behavioral evaluation was made 8 h after operation.According to different periods of time,8 h,12 h,1 d,3 d,5 d,7 d,21 d after operation,blood was taken from abdominal cavity and cerebrospinal fluid was taken to detect the level of S-100B protein and NSE.Brain staining and behavioral function of 2,3,5-triphenyltetrazolium chloride(TTC)were evaluated and their correlation was analyzed.Results The levels of S-100B and NSE in serum and cerebrospinal fluid of SD rats showed a trend change in different periods of time.The time of increasing the levels of S-100B and NSE in cerebrospinal fluid was earlier than the time of increasing the levels of S-100B and NSE in serum.12 h after brain injury,the levels of NSE in cerebrospinal fluid reached the peak,the levels of S-100B and NSE in serum increased,and reached the peak after 3 d,after 7 days,there was no significant difference in S-100B and NSE levels between the two groups(P>0.05)The levels of S-100B and NSE in serum and CSF were positively correlated with infarct area and neurological function score(P<0.05).Conclusion S-100B and NSE in cerebrospinal fluid and serum can reflect the degree of brain injury.As a biological index of ischemic stroke,it can further provide a time window for clinical patients to collect blood and cerebrospinal fluid samples.