风轮菜总黄酮联合miR-702-5p抑制物对缺氧/复氧诱导的心肌细胞损伤的影响

目的 研究风轮菜总黄酮联合miR-702-5p抑制物对缺氧/复氧诱导的心肌细胞损伤的影响.方法 实验设置对照组(Con,不作任何处理)、缺氧/复氧组(H/R,缺氧6 h,复氧3 h),H/R+药物-1组、H/R+药物-2组、H/R+药物-3组(6.25μg/ml、12.5μg/ml、25μg/ml的风轮菜总黄酮+缺氧/...

Effects of total flavonoids of Clinopodium chinense combined with miR-702-5p inhibitor on myocardial cell injury induced by hypoxia/reoxygenation

Objective To investigate the effects of total flavonoids of Clinopodium chinense combined with miR-702-5p inhibitor on myocardial cell injury induced by hypoxia/reoxygenation.Methods The experiment consisted of the control(Con)group(without any treatment),the hypoxia/reoxygenation(H/R)group(hypoxia for 6 h and reoxygenation for 3 h),the H/R+drug-1 group,the H/R+drug-2 group,H/R+Drug-3 group(6.25μg/ml,12.5μg/ml,25μg/ml total flavonoids of Clinopodium chinense+hypoxia/reoxygenation treatment),H/R+anti-miR-NC group(transfecting anti-miR-NC+hypoxia/reoxygenation treatment),H/R+anti-miR-702-5p group(transfecting anti-miR-702-5p+hypoxia/reoxygenation treatment),H/R+drug-3+anti-miR-NC group(transfecting anti-miR-NC+25μg/ml total flavonoids of Clinopodium chinense+hypoxia/reoxygenation treatment),H/R+drug-3+anti-miR-702-5p group(transfecting anti-miR-702-5p+25μg/ml total flavonoids of Clinopodium chinense+hypoxia/Reoxygenation).Western blot was used to detect the expression of cyclin-dependent kinase inhibitor 1A(P21),cysteine aspartase-3(Caspase-3)protein expression;cell counting kit 8(CCK-8)was used to detect cell survival rate;flow cytometry to detect apoptosis;kit was used to detect malondialdehyde(MDA)content and activity of lactate dehydrogenase(LDH),catalase(CAT),superoxide dismutase(SOD);real-time quantitative PCR(RT-qPCR)was used to detect miR-702-5p expression.Results In cardiomyocytes induced by hypoxia/reoxygenation,the expressions of P21 and Caspase-3 were increased,cell survival rate was decreased,cell apoptosis rate was increased,MDA content was increased,LDH activity was increased,and CAT and SOD activity were decreased.After treatment with different concentrations of total flavonoids of Clinopodium chinense,the expressions of P21 and Caspase-3 decreased,the cell survival rate was increased,the cell apoptosis rate was decreased,the MDA content was decreased,LDH activity was decreased,and CAT and SOD activity was increased.Inhibition of miR-702-5p expression can inhibit myocardial cell injury induced by hypoxia/reoxygenation.The total flavonoids of Clinopodium chinense combined with miR-702-5p inhibitor can significantly improve cell survival rate,inhibit apoptosis and oxidative stress response.Conclusion The total flavonoids of Clinopodium chinense combined with miR-702-5p inhibitor can protect myocardial cells from damage caused by hypoxia/reoxygenation.