| 成纤维细胞介导的G-CSF基因疗法促进化疗后造血功能恢复的实验研究 |
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| 引用本文： | 王全兴,曹雪涛,周正芳,章卫平,于益芝,孙延平,叶天星. 成纤维细胞介导的G-CSF基因疗法促进化疗后造血功能恢复的实验研究[J]. 中国肿瘤生物治疗杂志, 1996, 3(1): 33-37 |
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| 作者姓名： | 王全兴 曹雪涛 周正芳 章卫平 于益芝 孙延平 叶天星 |
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| 作者单位： | 第二军医大学免疫学教研室!上海200433 |
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| 基金项目： | 上海市科技启明星培养计划，国家自然科学基金，优秀中青年人才专项基金(39421009)资助 |
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| 摘 要： | 利用RT-PCR方法克隆到包括有全部编码序列和部分5′、3′端非编码序列的人G-CSF cDNA,并通过核苷酸测序得到证实。将其定向克隆至逆转录病毒载体pLXSN,构建成人G-CSF的重组逆转录病毒表达载体pLGSN。体外经CRE和CRIP细胞的两次包装,病毒滴度达到了临床应用的水平(1.1×10~6CFU/ml)。hG-CSF逆转录病毒感染NIH3T3小鼠成纤维细胞后,分泌G-CSF达168U/ml,Southern分析表明hG-CSF基因已整合至NIH3T3-G-CSF细胞的基因组中,Northern和Western分析分别从mRNA和蛋白质水平证实了人G-CSF在NIH3T3-G-CSF细胞的表达。将NIH3T3-G-CSF细胞植入同系小鼠体内,能从血清中检测到持续表达的G-CSF活性。本研究为开展人G-CSF基因治疗奠定了基础。
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| 关 键 词： | 粒细胞集落刺激因子 逆转录病毒 基因克隆 基因转移 基因治疗 序列测定 |
| 收稿时间： | 1995-10-21 |
The Enhancing Effects of Fibroblast-Mediated G-CSF Gene Therapy on the Recovery of Hematopoiesis |
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| Wang Quanxing,Cao Xuetao,Zhou Zhengfang,Zhang Weiping,Yu Yizhi,Sun Yanping and Ye Tianxing. The Enhancing Effects of Fibroblast-Mediated G-CSF Gene Therapy on the Recovery of Hematopoiesis[J]. Chinses Journal of Cancer Biotherapy, 1996, 3(1): 33-37 |
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| Authors: | Wang Quanxing Cao Xuetao Zhou Zhengfang Zhang Weiping Yu Yizhi Sun Yanping Ye Tianxing |
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| Affiliation: | Department of Immunology, Second Military Medical University, Shanghai 200433;Department of Immunology, Second Military Medical University, Shanghai 200433;Department of Immunology, Second Military Medical University, Shanghai 200433;Department of Immunology, Second Military Medical University, Shanghai 200433;Department of Immunology, Second Military Medical University, Shanghai 200433;Department of Immunology, Second Military Medical University, Shanghai 200433;Department of Immunology, Second Military Medical University, Shanghai 200433 |
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| Abstract: | In the present study, the effects of G-CSF gene therapy was investigated on the recovery of murine hematopoietic suppression induced by high dose of cyclophosphomide (Cy) . The results showed that G-CSF gene therapy could slow down the Cy-induced decreasing of peripheral WBCs and platelets, and accerelate their recovery.It also could increase the CFU-GM, CFU - MK, CFU-S derived from the splenocyte and bone marrow cells in the chemotherapy-treated mice. The data demonstrated that fibroblast-mediated G-CSF gene therapy could significantlyreduce the hematopoietic damage to less extent, and accerelate hematopoietic recovery after chemotherapy. |
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| Keywords: | G - CSF retrovirus gene cloning gene transfer gene therapy DNA sequencing |
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