微波和足叶乙甙诱导K562细胞的凋亡

目的：探讨微波和足叶乙甙体外诱导Ｋ５６２细胞凋亡和可能性及其机制。方法：将微波和足叶乙甙分别单位以及联合作用于正常骨髓细胞和Ｋ５６２细胞株，通过细胞凋亡率和ｂｃｌ－２和ｐ２１＾ｒａｓ蛋白阳性率的测定来评价细胞凋亡的程度并研究其机制。结果：微波和足叶乙甙各自都能诱导正常骨髓细胞和Ｋ５６２细胞的少量凋亡，两者联合作用所诱导的细胞凋亡率较之于足叶乙甙有显著性增加（Ｐ〈０．０５），其中Ｋ５６２细胞凋亡率的

K562 cell apoptosis induced by the combination of microwave and etoposide

Objective:To investigate the possibility and the mechanism of K562 apoptosis induced by microwave and etoposide Methods: Normal human bone marrow cell and K562 cell line were treated by microwave or etoposide alone, or by the combination of both The ratio of apoptotic cell (RAC) and the ratio of bcl 2 and p21ras proteins positive cell (RPC) were measured in order to evaluate cell apoptosis and analyze its mechanism Results: Both microwave and etoposide induced a little apoptosis of these two kinds of cells, while the combination of microwave and etoposide induced a remarkable higher RAC than that of etoposide alone (P<0 05) RAC increased much higher in K562 than that in normalhuman bone marrow cell RAC increase was simultaneously with RPC reduction Conclusion:The combination of microwave and etoposide could have a limited synergistic effect on inducing K562 apoptosis, which may be related to RPC reduction