姜黄素对血管性痴呆大鼠学习记忆的影响

目的：研究姜黄素对双侧颈总动脉永久性结扎（2VO）所致血管性痴呆大鼠学习记忆功能减退的作用。方法：以2VO来制备大鼠血管性痴呆模型，将大鼠分为3组（假手术组、溶剂对照组、给药组），一部分于术后2周检测脑组织氧自由基（O2^-）和羟自由基（OH），另一部分于术后4周和8周分别进行水迷宫实验，来检测各组大鼠学习记忆能力的差异，通过HE染色和免疫组化来观察细胞数量和形态的差异。结果：姜黄素能明显降低因2VO所造成的氧自由基（O2^-）和羟自由基（OH）水平的升高（P〈0．05），改善由2VO所致的学习记忆功能的减退（P〈0．05和P〈0．01）。HE染色和免疫组化证实，姜黄素减少了2VO所导致的神经细胞死亡。结论：姜黄素能改善2VO所致的学习记忆减退，其机制与减少自由基的产生以及减少神经细胞死亡有关。

Effects of curcumin on spatial learning and memory of vascular dementia rats

Objective: To investigate the effects of curcumin on spatial learning and memory of vascular dementia rats induced by a permanent bilateral ligation of common carotid arteries (2VO). Methods: SD rats were randomly divided into sham-operated, vehicle-treated and curcumin-treated group. The levels of O2.- and .OH were detected at 2 weeks after the surgery. The morris water maze test was performed to detect the effects of curcumin on spatial learning and memory of vascular dementia rats at 4 weeks and 8 weeks after the surgery. HE and immunocytochemistry were performed to investigate the cell number of CA1 subfeild of hippocampus. Results: Curcumin can significantly decrease the levels of O2.- and .OH. The spatial learning and memory of vascular dementia rats were impaired and that of the curcumin-treated group was ameliorated. HE and immunocytochemistry proved the difference of the cell number of CA1 subfield of hippocampus. Conclusion: The results demonstrate that curcumin can alleviate the impairment resulted from vascular dementia by reducing the level of free radical and the cell death of CA1 subfield of hippocampus.