Scintigraphy and distribution of labeled antibodies in rats with tumors |
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Authors: | J. Graham Sharp James W. Osborne Ph.D. H. F. Cheng K. L. Coop George R. Zimmerman |
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Affiliation: | (1) Radiation Research Laboratory (J.W.O., H.F.C., J.G.S., K.L.C.), University of Iowa, 52242 Iowa City, Iowa, USA;(2) Department of Radiology (H.F.C., J.W.O.), University of Iowa, 52242 Iowa City, Iowa, USA;(3) Burlington Memorial Hospital (G.R.Z.), 52601 Burlington, Iowa, USA;(4) Present address: Department of Anatomy, University of Nebraska Medical Center, 42nd and Dewey Avenues, 68105 Omaha, NE, USA;(5) Present address: Los Alamos Scientific Laboratory, Mail Stop 403, 87544 Los Alamos, NM, USA |
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Abstract: | Radioiodinated antitumor (Ab-gamma globulins), non-tumor-specific Ab, and R131ISA were used for imaging radiation-induced intestinal tumors in rats. Each agent detected tumors larger than 2 g, but labeled Ab were most efficient in detecting smaller tumors. Tissue distribution studies showed that while purified Ab localized specifically in tumors, unpurified Ab concentrated in the tumor by a mechanism not considered immunological. Localization was variable and the concentration of antitumor Ab reached useful levels only in a small number of cases. The use of high specific activity purified Ab unexpectedly decreased the concentrations of label observed in the tumors when compared with the use of the same activity of low specific activity purified Ab. These results indicated the presence of circulating tumor antigens which were capable of binding the injected Ab. Subsequently, these findings have been substantiated. Thus the animal-to-animal variability could be explained on the basis of differing degrees of interaction of injected Ab with circulating tumor antigens. The usefulness of labeled purified or monospecific antitumor antibodies for tumor imaging and therapy would thus be influenced by the extent of such interactions.This work was presented in part at the Twentieth Annual Meeting of the Radiation Research Society, Portland, Oregon, May, 1972. |
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