四氯化碳引起大鼠肝微粒体钙泵的抑制与巯基关系的探讨

四氯化碳（ＣＣｌ４）与肝微粒体共同温浴，引起微粒体的丙二醛（ＭＤＡ）升高、蛋白巯基的降低和钙泵的抑制。与对照组比较具有显著性意义，且存在着剂量－反应关系及时间－反应关系。在本实验条件下，发现Ｃｌ４引起蛋白巯基的降低和钙泵的抑制呈明显的相关关系。用巯基供剂ＤＴＴ、过化物自由基清除剂ＳＯＤ均能部分地拮抗ＣＣｌ４引起肝微粒体脂质过氧化（ＬＰＯ），从而保护了微粒体的蛋白巯基及钙泵。表现耿ＤＴＴ＋ＣＣｌ４及

Fu Liwu,et al.Tbe relationship of hepatic mi- crosomal calcium pumP and sulfhydryl content in ratsintoxicated by CCl_4

at liver microsomes incubated with carbon te-trachlorid(CCl_4 ) resulted in increase of malonvl-dialedehyde and decrease of protein sulfhydryl groupcontent and the activity of the calcium pump, Thechanges showed time-effect and dose-effect rela-tionship respectively and with significant differencesfrom that of control. The incubation of the micro-somes with CCl_4 and dithiothreitol(DTT) or supero-xide dismutase(SOD)simultaneously showed thatDTT and SOD could antagonize liper peroxidation(LPO)and restore the protein sulhydryl group andcalcium pump. In case of absence of LPO , more than80% of protein sulfhydryl group was specifically com-plexed by N-ethymaleimide(NEM), and the calciumpump was also inhibited. The results suggested thatthe calcium pump inhibited by CCI4 was associatedwith the reduction of protein sulfhydryl group due toLPO.